2j65: Difference between revisions
New page: left|200px<br /> <applet load="2j65" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j65, resolution 2.20Å" /> '''STRUCTURE OF LPXC F... |
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==About this Structure== | ==About this Structure== | ||
2J65 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]] with ZN, CL, MYR and UDP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J65 OCA]]. | 2J65 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]] with ZN, CL, MYR and UDP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J65 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: lipid synthesis]] | [[Category: lipid synthesis]] | ||
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Revision as of 11:49, 30 October 2007
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STRUCTURE OF LPXC FROM AQUIFEX AEOLICUS IN COMPLEX WITH UDP
OverviewOverview
The structure of recombinant Aquifex aeolicus, UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) in complex with UDP, has been determined to a resolution of 2.2 A. Previous studies have, characterized the binding sites of the fatty-acid and sugar moieties of, the substrate, UDP-(3-O-hydroxymyristoyl)-N-acetylglucosamine, but not, that of the nucleotide. The uracil-binding site is constructed from amino, acids that are highly conserved across species. Hydrophobic associations, with the Phe155 and Arg250 side chains in combination with, hydrogen-bonding interactions with the main chain of Glu154 and the side, chains of Tyr151 and Lys227 position the base. The phosphate and ribose, groups are directed away from the active site and interact with Arg137, Lys156, Glu186 and Arg250. The ... [(full description)]
About this StructureAbout this Structure
2J65 is a [Single protein] structure of sequence from [Aquifex aeolicus] with ZN, CL, MYR and UDP as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
The nucleotide-binding site of Aquifex aeolicus LpxC., Buetow L, Dawson A, Hunter WN, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1082-6. Epub 2006 Oct 25. PMID:17077484
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