2j5m: Difference between revisions
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[[Image:2j5m. | [[Image:2j5m.jpg|left|200px]]<br /><applet load="2j5m" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2j5m" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2j5m, resolution 1.75Å" /> | caption="2j5m, resolution 1.75Å" /> | ||
'''STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0'''<br /> | '''STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2J5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, ACT, HEM, PEO and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | 2J5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, ACT, HEM, PEO and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Act Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J5M OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:49:46 2007'' | ||
Revision as of 20:39, 18 December 2007
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STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0
OverviewOverview
We have determined the crystal structure of the chloroperoxidase (CPO), hydroperoxo reaction intermediate (CPO compound 0) at 1.75-A resolution., The intermediate was generated through controlled photoreduction of the, CPO oxygen complex during x-ray data collection, which was monitored by, recording of the crystal absorption spectra. Initially, the peroxo-anion, species was formed and then protonated to yield compound 0. Quantum, chemical calculations indicate that the peroxo-anion species is not stable, and collapses instantaneously to compound 0. Compound 0 is present in the, ferric low-spin doublet ground state and is characterized by a long O O, bond length of 1.5 A and a Fe O bond distance of 1.8 A, which is also, observed in the crystal structure.
About this StructureAbout this Structure
2J5M is a Single protein structure of sequence from Leptoxyphium fumago with NAG, MAN, MN, ACT, HEM, PEO and EDO as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes., Kuhnel K, Derat E, Terner J, Shaik S, Schlichting I, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):99-104. Epub 2006 Dec 26. PMID:17190816
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