2j4d: Difference between revisions
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[[Image:2j4d. | [[Image:2j4d.jpg|left|200px]]<br /><applet load="2j4d" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2j4d" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2j4d, resolution 1.9Å" /> | caption="2j4d, resolution 1.9Å" /> | ||
'''CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA'''<br /> | '''CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2J4D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with FAD and MHF as [http://en.wikipedia.org/wiki/ligands ligands]. | 2J4D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with FAD and MHF as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mhf Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4D OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transit peptide]] | [[Category: transit peptide]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:48:05 2007'' | ||
Revision as of 20:38, 18 December 2007
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CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA
OverviewOverview
Cryptochromes are almost ubiquitous blue-light receptors and act in, several species as central components of the circadian clock. Despite, being evolutionary and structurally related with DNA photolyases, a class, of light-driven DNA-repair enzymes, and having similar cofactor, compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from, the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its, crystal structure determined at 1.9 Angstroms resolution shows, cryptochrome 3 in a dimeric state with the antenna cofactor, 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms, to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a, residue crucial for MTHF-binding, E149, was replaced by site-directed, mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional, antenna for the photoreduction of FAD.
About this StructureAbout this Structure
2J4D is a Single protein structure of sequence from Arabidopsis thaliana with FAD and MHF as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna., Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO, J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299
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