2ixc: Difference between revisions
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[[Image:2ixc.gif|left|200px]]<br /> | [[Image:2ixc.gif|left|200px]]<br /><applet load="2ixc" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2ixc" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2ixc, resolution 1.79Å" /> | caption="2ixc, resolution 1.79Å" /> | ||
'''RMLC M. TUBERCULOSIS WITH DTDP-RHAMNOSE'''<br /> | '''RMLC M. TUBERCULOSIS WITH DTDP-RHAMNOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2IXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with TRH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/dTDP-4-dehydrorhamnose_3,5-epimerase dTDP-4-dehydrorhamnose 3,5-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.13 5.1.3.13] | 2IXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with TRH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/dTDP-4-dehydrorhamnose_3,5-epimerase dTDP-4-dehydrorhamnose 3,5-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.13 5.1.3.13] Known structural/functional Site: <scene name='pdbsite=AC1:Trh Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IXC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: lipopolysaccharide biosynthesis]] | [[Category: lipopolysaccharide biosynthesis]] | ||
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Revision as of 20:29, 18 December 2007
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RMLC M. TUBERCULOSIS WITH DTDP-RHAMNOSE
OverviewOverview
The striking feature of carbohydrates is their constitutional, conformational and configurational diversity. Biology has harnessed this, diversity and manipulates carbohydrate residues in a variety of ways, one, of which is epimerization. RmlC catalyzes the epimerization of the C3' and, C5' positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming, dTDP-6-deoxy-L-lyxo-4-hexulose. RmlC is the third enzyme of the rhamnose, pathway, and represents a validated anti-bacterial drug target. Although, several structures of the enzyme have been reported, the mechanism and the, nature of the intermediates have remained obscure. Despite its relatively, small size (22 kDa), RmlC catalyzes four stereospecific proton transfers, and the substrate undergoes a major conformational change during the, course of the transformation. Here we report the structure of RmlC from, several organisms in complex with product and product mimics. We have, probed site-directed mutants by assay and by deuterium exchange. The, combination of structural and biochemical data has allowed us to assign, key residues and identify the conformation of the carbohydrate during, turnover. Clear knowledge of the chemical structure of RmlC reaction, intermediates may offer new opportunities for rational drug design.
About this StructureAbout this Structure
2IXC is a Single protein structure of sequence from Mycobacterium tuberculosis with TRH as ligand. Active as dTDP-4-dehydrorhamnose 3,5-epimerase, with EC number 5.1.3.13 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation., Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH, J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787
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