2iu4: Difference between revisions
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[[Image:2iu4.gif|left|200px]]<br /> | [[Image:2iu4.gif|left|200px]]<br /><applet load="2iu4" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2iu4" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2iu4, resolution 1.96Å" /> | caption="2iu4, resolution 1.96Å" /> | ||
'''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''<br /> | '''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2IU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with SO4 and HIQ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] | 2IU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with SO4 and HIQ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 20:24, 18 December 2007
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DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ
OverviewOverview
Dihydroxyacetone (Dha) kinases are a novel family of kinases with, signaling and metabolic functions. Here we report the x-ray structures of, the transcriptional activator DhaS and the coactivator DhaQ and, characterize their function. DhaQ is a paralog of the Dha binding Dha, kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional, activator. DhaQ and DhaS form a stable complex that in the presence of Dha, activates transcription of the Lactococcus lactis dha operon. Dha, covalently binds to DhaQ through a hemiaminal bond with a histidine and, thereby induces a conformational change, which is propagated to the, surface via a cantilever-like structure. DhaS binding protects an inverted, repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs, of the operator DNA hypersensitive to DNase I cleavage. The proximal, half-site of the inverted repeat partially overlaps with the predicted -35, consensus sequence of the dha promoter.
About this StructureAbout this Structure
2IU4 is a Single protein structure of sequence from Lactococcus lactis with SO4 and HIQ as ligands. Active as Glucokinase, with EC number 2.7.1.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471
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