2c39: Difference between revisions
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[[Image:2c39.gif|left|200px]]<br /> | [[Image:2c39.gif|left|200px]]<br /><applet load="2c39" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2c39" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2c39, resolution 3.30Å" /> | caption="2c39, resolution 3.30Å" /> | ||
'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP'''<br /> | '''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2C39 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. | 2C39 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Adp Binding Site For Chain X'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C39 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rrp42]] | [[Category: rrp42]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:07:49 2007'' | ||
Revision as of 19:58, 18 December 2007
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RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP
OverviewOverview
The exosome is a macromolecular complex that plays fundamental roles in, the biogenesis and turnover of a large number of RNA species. Here we, report the crystal structures of the Rrp41-Rrp42 core complex of the S., solfataricus exosome bound to short single-stranded RNAs and to ADP. The, RNA binding cleft recognizes four nucleotides in a sequence-unspecific, manner, mainly by electrostatic interactions with the phosphate groups., Interactions at the 2' hydroxyls of the sugars provide specificity for RNA, over DNA. The structures show both the bound substrate and the cleaved, product of the reaction, suggesting a catalytic mechanism for the 3'-5', phosphorolytic activity of the exosome.
About this StructureAbout this Structure
2C39 is a Protein complex structure of sequences from Sulfolobus solfataricus with ADP as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928
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