2bt3: Difference between revisions

Revision as of 19:48, 18 December 2007

AGAO IN COMPLEX WITH RUTHENIUM-C4-WIRE AT 1.73 ANGSTROMS

OverviewOverview

Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an, aromatic tail group, and an alkane linker reversibly inhibit the activity, of the copper amine oxidase from Arthrobacter globiformis (AGAO), with, K(i) values between 6 muM and 37 nM. In the crystal structure of a, Ru(II)-wire:AGAO conjugate, the wire occupies the AGAO active-site, substrate access channel, the trihydroxyphenylalanine quinone cofactor is, ordered in the "off-Cu" position with its reactive carbonyl oriented, toward the inhibitor, and the "gate" residue, Tyr-296, is in the "open", position. Head groups, tail-group substituents, and linker lengths all, influence wire-binding interactions with the enzyme.

About this StructureAbout this Structure

2BT3 is a Single protein structure of sequence from Arthrobacter globiformis with CU, NA, SO4, R4A and GOL as ligands. Active as Amine oxidase (copper-containing), with EC number 1.4.3.6 Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

Reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(II) and rhenium(I) molecular wires., Contakes SM, Juda GA, Langley DB, Halpern-Manners NW, Duff AP, Dunn AR, Gray HB, Dooley DM, Guss JM, Freeman HC, Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13451-6. Epub 2005 Sep 12. PMID:16157884

Page seeded by OCA on Tue Dec 18 18:57:49 2007

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OCA

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-[[Image:2bt3.gif|left|200px]]<br />
+[[Image:2bt3.gif|left|200px]]<br /><applet load="2bt3" size="450" color="white" frame="true" align="right" spinBox="true"
-<applet load="2bt3" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2bt3, resolution 1.73&Aring;" />
 '''AGAO IN COMPLEX WITH RUTHENIUM-C4-WIRE AT 1.73 ANGSTROMS'''<br />
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 ==About this Structure==
-BT3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with CU, NA, SO4, R4A and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BT3 OCA].
+BT3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with CU, NA, SO4, R4A and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BT3 OCA].
 ==Reference==
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 [[Category: tpq]]
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