1uuw: Difference between revisions

Revision as of 19:03, 18 December 2007

NAPHTHALENE 1,2-DIOXYGENASE WITH NITRIC OXIDE BOUND IN THE ACTIVE SITE.

OverviewOverview

Nitric oxide (NO) is commonly used as an analogue for dioxygen in, structural and spectroscopic studies of oxygen binding and oxygen, activation. In this study, crystallographic structures of naphthalene, dioxygenase (NDO) in complex with nitric oxide are reported. In the, presence of the aromatic substrate indole, NO is bound end-on to the, active-site mononuclear iron of NDO. The structural observations correlate, well with spectroscopic measurements of NO binding to NDO in solution., However, the end-on binding of NO is in contrast to the recently reported, structure of oxygen to the active-site iron of NDO that binds side-on., While NO is a good oxygen analogue with many similarities to O(2), the, different binding mode of NO to the active-site iron atom leads to, different mechanistic implications. Hence, caution needs to be used in, extrapolating NO as an analogue to O(2) binding.

About this StructureAbout this Structure

1UUW is a Protein complex structure of sequences from Pseudomonas putida with FE, SO4, FES and NO as ligands. Active as Naphthalene 1,2-dioxygenase, with EC number 1.14.12.12 Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

NO binding to naphthalene dioxygenase., Karlsson A, Parales JV, Parales RE, Gibson DT, Eklund H, Ramaswamy S, J Biol Inorg Chem. 2005 Aug;10(5):483-9. Epub 2005 Sep 23. PMID:15942729

Page seeded by OCA on Tue Dec 18 18:12:54 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 '''NAPHTHALENE 1,2-DIOXYGENASE WITH NITRIC OXIDE BOUND IN THE ACTIVE SITE.'''<br />
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 ==About this Structure==
-UUW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE, SO4, FES and NO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UUW OCA].
+UUW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE, SO4, FES and NO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UUW OCA].
 ==Reference==
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