Molecular Playground/Caspase-7 Dynamics: Difference between revisions

Revision as of 21:16, 3 May 2010

Caspases are a family of CBI Molecules being studied in the University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst and on display at the Molecular Playground.

Conformation Dynamics studied in the Hardy Lab Conformation Dynamics studied in the Hardy Lab

Conformational Dynamics between active and allosterically inhibited caspase-7 elucidate the mechanism of allostery in this important class of cysteine proteases.

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Conformational dynamics in Caspase-7 are mediated by an 'Allosteric Toggle' mechanism in which binding of allosteric inhibitor DICA to CYS 290 pushes TYR 223 into 'up' conformation forcing ARG 187 'out' into a form that is physically incompatible with substrate binding.

The cleaved termini of the large and small subunits which form the active site loop bundle become highly ordered in active conformation, and highly disordered in allosterically inhibited form (so much so that they cannot be resolved crystallographically).

Forms of Caspase-7Forms of Caspase-7

, trapping protein in active/substrate bound conformation.
trapping protein in a form incompatible with substrate binding.
of Caspase-7.

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Molecular Playground banner: Conformational Dynamics between active and allosterically inhibited caspase-7 elucidate the mechanism of allostery in this important class of cysteine proteases.

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Daniel Seeman, David Canner, Michal Harel, Maureen E. Hill

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 === Forms of Caspase-7 ===
-*<scene name='User:Daniel_Seeman/Caspase-7_Dynamics/1f1j/1'>Caspase-7 bound to suicide inhibitor/substrate mimic DEVD-CHO</scene>, trapping protein in active/substrate bound conformation.
+*<scene name='Molecular_Playground/Caspase_Dynamics/1f1j/2'>Caspase-7 bound to suicide inhibitor/substrate mimic DEVD-CHO</scene>, trapping protein in active/substrate bound conformation.
 *<scene name='User:Daniel_Seeman/Caspase-7_Dynamics/1shj-234234/1'>Caspase-7 bound to allosteric inhibitor DICA through CYS290</scene> trapping protein in a form incompatible with substrate binding.
 *<scene name='Molecular_Playground/Caspase_Dynamics/Morph2/1'>Conformational change between substrate bound and substrate incompatible forms</scene> of Caspase-7.