1ump: Difference between revisions
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[[Image:1ump.gif|left|200px]]<br /> | [[Image:1ump.gif|left|200px]]<br /><applet load="1ump" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1ump" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1ump, resolution 2.13Å" /> | caption="1ump, resolution 2.13Å" /> | ||
'''GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE'''<br /> | '''GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1UMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with C8E and SQA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Squalene--hopene_cyclase Squalene--hopene cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.17 5.4.99.17] | 1UMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with C8E and SQA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Squalene--hopene_cyclase Squalene--hopene cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.17 5.4.99.17] Known structural/functional Site: <scene name='pdbsite=AC1:Sqa Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UMP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: triterpene cyclase]] | [[Category: triterpene cyclase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:03:31 2007'' | ||
Revision as of 18:53, 18 December 2007
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GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE
OverviewOverview
The membrane protein squalene-hopene cyclase was cocrystallized with, 2-azasqualene and analyzed by X-ray diffraction to 2.13 A resolution. The, conformation of this close analog was clearly established, and it agreed, with the common textbook presentation. The bound squalene undergoes only, small conformational changes during the formation of rings A through D, thus requiring no intermediate. However, ring E formation is hindered by, an entropic barrier, which may explain its absence in the steroids. The, structure analysis revealed a mobile region between the active center, cavity and the membrane, which may melt, opening a passage for squalene, and hopene.
About this StructureAbout this Structure
1UMP is a Single protein structure of sequence from Alicyclobacillus acidocaldarius with C8E and SQA as ligands. Active as Squalene--hopene cyclase, with EC number 5.4.99.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Conversion of squalene to the pentacarbocyclic hopene., Reinert DJ, Balliano G, Schulz GE, Chem Biol. 2004 Jan;11(1):121-6. PMID:15113001
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