1scj: Difference between revisions
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[[Image:1scj.gif|left|200px]]<br /> | [[Image:1scj.gif|left|200px]]<br /><applet load="1scj" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1scj" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1scj, resolution 2.00Å" /> | caption="1scj, resolution 2.00Å" /> | ||
'''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX'''<br /> | '''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1SCJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] | 1SCJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] Known structural/functional Site: <scene name='pdbsite=AVE:Active Site Mutation Is SER 221 CYS That Blocks The Prot ...'>AVE</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SCJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: subtilisin e - propeptide]] | [[Category: subtilisin e - propeptide]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:59:49 2007'' | ||
Revision as of 18:50, 18 December 2007
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CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX
OverviewOverview
We report here the crystallographic structure determination of an, autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A, resolution. The subtilisin domain sequence has a single substitution, (Ser221Cys) which has been shown to block the maturation process prior to, degradation of the propeptide domain (77 residues) that acts as an, intramolecular chaperon. This mutation, however, did not prevent the, enzyme from cleaving its propeptide domain with a 60-80% efficiency. The, current determination is the first example of a subtilisin E-propeptide, complex which has been autoprocessed. A previous structure determination, of a BPN'-prosegment complex has been reported in which the subtilisin, domain was extensively mutated and a calcium binding loop was deleted., Further, in this earlier determination, the complex was formed by the, addition of separately expressed propeptide domain. The structure, determination reported here provides additional information about the, nature of the interaction between the subtilisin and propeptide domains in, this complex.
About this StructureAbout this Structure
1SCJ is a Protein complex structure of sequences from Bacillus subtilis with CA as ligand. Active as Subtilisin, with EC number 3.4.21.62 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:9811547
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