1qno: Difference between revisions
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[[Image:1qno.gif|left|200px]]<br /> | [[Image:1qno.gif|left|200px]]<br /><applet load="1qno" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1qno" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1qno, resolution 2.00Å" /> | caption="1qno, resolution 2.00Å" /> | ||
'''THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5'''<br /> | '''THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1QNO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina] with NAG and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] | 1QNO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina] with NAG and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Known structural/functional Site: <scene name='pdbsite=MAN:Mannan Binding Site'>MAN</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: trichoderma reesei]] | [[Category: trichoderma reesei]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:57:50 2007'' | ||
Revision as of 18:48, 18 December 2007
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THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5
OverviewOverview
The crystal structure of the catalytic core domain of beta-mannanase from, the fungus Trichoderma reesei has been determined at a resolution of 1.5, A. The structure was solved using the anomalous scattering from a single, non-isomorphous platinum complex with two heavy-metal sites in space group, P2(1). The map computed with the experimental phases was enhanced by the, application of an automated model building and refinement procedure using, the amplitudes and experimental phases as observations. This approach is, expected to be of more general application. The structure of the native, enzyme and complexes with Tris-HCl and mannobiose are also reported: the, mannobiose binds in subsites +1 and +2. The structure is briefly compared, with that of the homologous beta-mannanase from the bacterium, Thermomonospora fusca.
About this StructureAbout this Structure
1QNO is a Single protein structure of sequence from Hypocrea jecorina with NAG and TRS as ligands. Active as Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5., Sabini E, Schubert H, Murshudov G, Wilson KS, Siika-Aho M, Penttila M, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):3-13. PMID:10666621
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