Triose Phosphate Isomerase: Difference between revisions
Gregg Snider (talk | contribs) |
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== Mechanism == | == Mechanism == | ||
TPI catalyzes the transfer of a hydrogen atom from carbon 1 to carbon 2, an intramolecular [http://en.wikipedia.org/wiki/Oxidation_reduction oxidation-reduction] reaction. This isomerization of a ketose to an aldose proceeds through an cis-enediol intermediate. This isomerization proceeds without any cofactors and the enzyme confers a 10<sup>9</sup> rate enhancement relative to the nonenzymatic reaction involving a chemical base (acetate ion).<ref>PMID:2043623</ref> | TPI catalyzes the transfer of a hydrogen atom from carbon 1 to carbon 2, an intramolecular [http://en.wikipedia.org/wiki/Oxidation_reduction oxidation-reduction] reaction. This isomerization of a ketose to an aldose proceeds through an ''cis''-enediol(ate) intermediate. This isomerization proceeds without any cofactors and the enzyme confers a 10<sup>9</sup> rate enhancement relative to the nonenzymatic reaction involving a chemical base (acetate ion).<ref>PMID:2043623</ref> | ||
[[Image:kinetics2.png|center|thumb|400px| '''Kinetic constants of Triose Phosphate Isomerase''']] | [[Image:kinetics2.png|center|thumb|400px| '''Kinetic constants of Triose Phosphate Isomerase''']] | ||