User:Cameron Evans/Sandbox 1: Difference between revisions

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Glutamate Dehydrogenase (GluDH) is a member of the superfamily of amino acid dehydrogenase and functions in the cell to dehydrate α-ketoglutarate to the amino acid glutamate and also to perform the reverse reaction.<ref name="1bgv">PMID:8263917</ref>

GluDH is at the threshold of carbon metabolism (GluDH feeds α-ketoglutarate into the tricarboxylic acid cycle) and nitrogen metabolism (the amine product is utilized by other biosynthetic pathways).<ref name="1hwxyz">PMID:11254391</ref>. Due to its prominent position on the threshold between catabolic and anabolic pathways, GluDH is ubiquitously expressed in both complex and simple organisms.<ref name="1hwx">PMID:10425679</ref>

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Image:Alpha-ketoglutamate2.png |[[α-Ketoglutaraic acid]]

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GluDH is at the threshold of carbon metabolism (GluDH feeds α-ketoglutarate into the tricarboxylic acid cycle) and nitrogen metabolism (the amine product is utilized by other biosynthetic pathways).<ref name="1hwxyz">PMID:11254391</ref>. Due to its prominent position on the threshold between catabolic and anabolic pathways, GluDH is ubiquitously expressed in both complex and simple organisms.<ref name="1hwx">PMID:10425679</ref>

In vertebrates the produced ammonia is usually utilized in the urea cycle and in bacteria the ammonia is assimilated to amino acids and amidotransferases.<ref name="Lightfoot_1988">{{cite journal | author = Lightfoot DA, Baron AJ, Wootton JC | year = 1988 | title = Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance | journal = Plant Molecular Biology | volume = 11 | issue = 3 | pages = 335-344 | doi = 10.1007/BF00027390 }}</ref>

Reductive amination of α-ketoglutarate (α-KG) is the process by which the ketone is converted to an amine via an imine intermediate. The reverse reaction, oxidative deamination, is the conversion of the amine functional group to a ketone.

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 Glutamate Dehydrogenase (GluDH) is a member of the superfamily of amino acid dehydrogenase and functions in the cell to dehydrate α-ketoglutarate to the amino acid glutamate and also to perform the reverse reaction.<ref name="1bgv">PMID:8263917</ref>
-GluDH is at the threshold of carbon metabolism (GluDH feeds α-ketoglutarate into the tricarboxylic acid cycle) and nitrogen metabolism (the amine product is utilized by other biosynthetic pathways).<ref name="1hwxyz">PMID:11254391</ref>. Due to its prominent position on the threshold between catabolic and anabolic pathways, GluDH is ubiquitously expressed in both complex and simple organisms.<ref name="1hwx">PMID:10425679</ref>
 <gallery>
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   Image:Alpha-ketoglutamate2.png |[[α-Ketoglutaraic acid]]
 </gallery>
+GluDH is at the threshold of carbon metabolism (GluDH feeds α-ketoglutarate into the tricarboxylic acid cycle) and nitrogen metabolism (the amine product is utilized by other biosynthetic pathways).<ref name="1hwxyz">PMID:11254391</ref>. Due to its prominent position on the threshold between catabolic and anabolic pathways, GluDH is ubiquitously expressed in both complex and simple organisms.<ref name="1hwx">PMID:10425679</ref>
+In vertebrates the produced ammonia is usually utilized in the urea cycle and in bacteria the ammonia is assimilated to amino acids and amidotransferases.<ref name="Lightfoot_1988">{{cite journal | author = Lightfoot DA, Baron AJ, Wootton JC | year = 1988 | title = Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance | journal = Plant Molecular Biology | volume = 11 | issue = 3 | pages = 335-344 | doi = 10.1007/BF00027390 }}</ref>
 Reductive amination of α-ketoglutarate (α-KG) is the process by which the ketone is converted to an amine via an imine intermediate. The reverse reaction, oxidative deamination, is the conversion of the amine functional group to a ketone.