1dym: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
1DYM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ | 1DYM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Site: ACI. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYM OCA]]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate., MacKenzie LF, Sulzenbacher G, Divne C, Jones TA, Woldike HF, Schulein M, Withers SG, Davies GJ, Biochem J. 1998 Oct 15;335 ( Pt 2):409-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761741 9761741] | Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate., MacKenzie LF, Sulzenbacher G, Divne C, Jones TA, Woldike HF, Schulein M, Withers SG, Davies GJ, Biochem J. 1998 Oct 15;335 ( Pt 2):409-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761741 9761741] | ||
[[Category: Cellulase]] | |||
[[Category: Humicola insolens]] | [[Category: Humicola insolens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:33:30 2007'' | ||
Revision as of 11:28, 30 October 2007
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HUMICOLA INSOLENS ENDOCELLULASE CEL7B (EG 1) E197A MUTANT ENDOGLUCANASE, HYDROLASE, CELLULASE, CELLULOSE DEGRADATION, GLYCOSIDE HYDROLASE FAMILY 7, GLYCOSYNTHASE
OverviewOverview
Cellulose is the major polysaccharide component of the plant cell wall and, the most abundant naturally produced macromolecule on Earth. The enzymic, degradation of cellulose, by cellulases, is therefore of great, environmental and commercial significance. Cellulases are found in 12 of, the glycoside hydrolase families classified according to their amino acid, sequence similarities. Endoglucanase I (Cel7B), from the soft-rot fungus, Humicola insolens, is a family 7 enzyme. The structure of the native form, of Cel7B from H. insolens at 2.2 A resolution has been solved by molecular, replacement using the known Trichoderma reesei cellobiohydrolase I [Divne, Stahlberg, Reinikainen, Ruohonen, Pettersson, Knowles, Teeri and Jones, (1994) Science 265, 524-528] structure as the search model. ... [(full description)]
About this StructureAbout this Structure
1DYM is a [Single protein] structure of sequence from [Humicola insolens] with NAG as [ligand]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Site: ACI. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate., MacKenzie LF, Sulzenbacher G, Divne C, Jones TA, Woldike HF, Schulein M, Withers SG, Davies GJ, Biochem J. 1998 Oct 15;335 ( Pt 2):409-16. PMID:9761741
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