1hl7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1hl7.gif|left|200px]]<br /> | [[Image:1hl7.gif|left|200px]]<br /><applet load="1hl7" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1hl7" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1hl7, resolution 1.73Å" /> | caption="1hl7, resolution 1.73Å" /> | ||
'''GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE'''<br /> | '''GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1HL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Microbacterium Microbacterium] with BD1 as [http://en.wikipedia.org/wiki/ligand ligand]. | 1HL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Microbacterium Microbacterium] with BD1 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Bd1 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HL7 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 22: | Line 21: | ||
[[Category: tetrahedral intermediate]] | [[Category: tetrahedral intermediate]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:32:09 2007'' | ||
Revision as of 17:22, 18 December 2007
|
GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE
OverviewOverview
The structure of the recombinant (-) gamma-lactamase from an, Aureobacterium species has been solved at 1.73A resolution in the cubic, space group F23 with unit cell parameters a=b=c=240.6A. The trimeric, enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor, free haloperoxidases. The structure has been solved in complex with a, covalently bound ligand originating from the host cell and also in the, unligated form. The associated density in the former structure has been, interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo, [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the, catalytic Ser98. Soaks of these crystals with the industrial substrate, gamma-lactam or its structural analogue, norcamphor, result in the, displacement of the ligand from the enzyme active site, thereby allowing, determination of the unligated structure. The presence of the ligand in, the active site protects the enzyme from serine hydrolase inhibitors., Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a, substrate of the enzyme.
About this StructureAbout this Structure
1HL7 is a Single protein structure of sequence from Microbacterium with BD1 as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810
Page seeded by OCA on Tue Dec 18 16:32:09 2007