1h5k: Difference between revisions
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[[Image:1h5k. | [[Image:1h5k.jpg|left|200px]]<br /><applet load="1h5k" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1h5k" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1h5k, resolution 1.60Å" /> | caption="1h5k, resolution 1.60Å" /> | ||
'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (78-89% DOSE)'''<br /> | '''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (78-89% DOSE)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1H5K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana] with ACT, CA, HEM and PEO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] | 1H5K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana] with ACT, CA, HEM and PEO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] Known structural/functional Site: <scene name='pdbsite=CAA:Hem Binding Site For Chain A'>CAA</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5K OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: x-ray induced reduction]] | [[Category: x-ray induced reduction]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:02:15 2007'' | ||
Revision as of 16:52, 18 December 2007
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X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (78-89% DOSE)
OverviewOverview
A molecular description of oxygen and peroxide activation in biological, systems is difficult, because electrons liberated during X-ray data, collection reduce the active centres of redox enzymes catalysing these, reactions. Here we describe an effective strategy to obtain crystal, structures for high-valency redox intermediates and present a, three-dimensional movie of the X-ray-driven catalytic reduction of a bound, dioxygen species in horseradish peroxidase (HRP). We also describe, separate experiments in which high-resolution structures could be obtained, for all five oxidation states of HRP, showing such structures with, preserved redox states for the first time.
About this StructureAbout this Structure
1H5K is a Single protein structure of sequence from Armoracia rusticana with ACT, CA, HEM and PEO as ligands. Active as Peroxidase, with EC number 1.11.1.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:12024218
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