1h0m: Difference between revisions
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[[Image:1h0m.gif|left|200px]]<br /> | [[Image:1h0m.gif|left|200px]]<br /><applet load="1h0m" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1h0m" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1h0m, resolution 3.00Å" /> | caption="1h0m, resolution 3.00Å" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF THE QUORUM SENSING PROTEIN TRAR BOUND TO ITS AUTOINDUCER AND TO ITS TARGET DNA'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF THE QUORUM SENSING PROTEIN TRAR BOUND TO ITS AUTOINDUCER AND TO ITS TARGET DNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1H0M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens] with HSL as [http://en.wikipedia.org/wiki/ligand ligand]. | 1H0M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens] with HSL as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AA1:Hsl And Ooa Binding Site For Chain D'>AA1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H0M OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: trar]] | [[Category: trar]] | ||
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Revision as of 16:40, 18 December 2007
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THREE-DIMENSIONAL STRUCTURE OF THE QUORUM SENSING PROTEIN TRAR BOUND TO ITS AUTOINDUCER AND TO ITS TARGET DNA
OverviewOverview
The quorum sensing system allows bacteria to sense their cell density and, initiate an altered pattern of gene expression after a sufficient quorum, of cells has accumulated. In Agrobacterium tumefaciens, quorum sensing, controls conjugal transfer of the tumour- inducing plasmid, responsible, for plant crown gall disease. The core components of this system are the, transcriptional regulator TraR and its inducing ligand, N-(3-oxo-octanoyl)-L-homoserine lactone. This complex binds DNA and, activates gene expression. We have determined the crystal structure of, TraR in complex with its autoinducer and target DNA (PDB code 1h0m). The, protein is dimeric, with each monomer composed of an N-terminal domain, which binds the ligand in an enclosed cavity far from the dimerization, region, and a C-terminal domain, which binds DNA via a helix-turn-helix, motif. The structure reveals an asymmetric homodimer, with one monomer, longer than the other. The N-terminal domain resembles GAF/PAS domains, normally fused to catalytic signalling domains. In TraR, the gene fusion, is between a GAF/PAS domain and a DNA-binding domain, resulting in a, specific transcriptional regulator involved in quorum sensing.
About this StructureAbout this Structure
1H0M is a Protein complex structure of sequences from Agrobacterium tumefaciens with HSL as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA., Vannini A, Volpari C, Gargioli C, Muraglia E, Cortese R, De Francesco R, Neddermann P, Marco SD, EMBO J. 2002 Sep 2;21(17):4393-401. PMID:12198141
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