Jasper Lactate Final: Difference between revisions
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The <scene name='Jasper_Lactate_Final/Secondary/1'>secondary structure</scene> of LDH as shown here is comprised of 40% alpha helices and 23% beta sheets.(2) The SCOP data classifies this form of lactate dehydrogenase as mixed beta-alpha-beta, with mainly parallel beta sheets. | The <scene name='Jasper_Lactate_Final/Secondary/1'>secondary structure</scene> of LDH as shown here is comprised of 40% alpha helices and 23% beta sheets.(2) The SCOP data classifies this form of lactate dehydrogenase as mixed beta-alpha-beta, with mainly parallel beta sheets. | ||
==Kinetics== | |||
Kinetic studies of lactate dehydrogenase with oxalate and oxamate (structural analogues of lactate and pyruvate)have proven the mechanism stated above. The rate limiting step in this reaction is the rate of dissociation of NAD+ and NADH. The conversion of pyruvate to lactate with the subsequent regeneration of NAD+ is very favorable(1). | |||
==Catalysis== | ==Catalysis== | ||
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[[Image:2nd.png|left|thumb|355px|(1)]] | [[Image:2nd.png|left|thumb|355px|(1)]] | ||