Cory Tiedeman Sandbox 1: Difference between revisions
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==Mechanism== | ==Mechanism== | ||
[[Image:mechanism.png|left|400px]]<ref>{{website2}}</ref> | [[Image:mechanism.png|left|400px|The mechanism of 2PG to PEP using enolase.]]<ref>{{website2}}</ref> | ||
The | The | ||
<scene name='Cory_Tiedeman_Sandbox_1/Active_site/1'>active site</scene> of enolase as shown, involves Lys 345, Lys 396, Glu 168, Glu 211, and His 159. Enolase forms a complex with two | <scene name='Cory_Tiedeman_Sandbox_1/Active_site/1'>active site</scene> of enolase as shown, involves Lys 345, Lys 396, Glu 168, Glu 211, and His 159. Enolase forms a complex with two | ||
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==Kinetics== | ==Kinetics== | ||
[[Image:enolase kinetics.jpeg|left|150px]]<ref>{{journal2}}</ref> | [[Image:enolase kinetics.jpeg|left|150px|V vs. [PGA]; PGA is 2PG, the top curve has [Mg2+] of 10^-3 M and the bottom curve has [Mg2+] of 106-2 M]]<ref>{{journal2}}</ref> | ||
Since Mg2+ is essential for binding the substrate, 2-PG, it is also needed at a specific quality in order to have a good rate, or velocity. The graph shows the V vs. [PGA], in which PGA is 2-PG, with two different concentrations of Mg2+. The upper curve, which also has greater Vmax, has an Mg2+ concentration of 10^-3 M while the lower curve, which has a lower Vmax, has an Mg2+ concentration of 10^-2 M<ref>{{journal2}}</ref>. The Km is also larger the upper curve making the higher [Mg2+] more desirable. | Since Mg2+ is essential for binding the substrate, 2-PG, it is also needed at a specific quality in order to have a good rate, or velocity. The graph shows the V vs. [PGA], in which PGA is 2-PG, with two different concentrations of Mg2+. The upper curve, which also has greater Vmax, has an Mg2+ concentration of 10^-3 M while the lower curve, which has a lower Vmax, has an Mg2+ concentration of 10^-2 M<ref>{{journal2}}</ref>. The Km is also larger the upper curve making the higher [Mg2+] more desirable. | ||