1gsp: Difference between revisions
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[[Image:1gsp.gif|left|200px]]<br /> | [[Image:1gsp.gif|left|200px]]<br /><applet load="1gsp" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1gsp" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1gsp, resolution 2.20Å" /> | caption="1gsp, resolution 2.20Å" /> | ||
'''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY'''<br /> | '''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1GSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and SGP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] | 1GSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and SGP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Known structural/functional Sites: <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active Site'>CAT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GSP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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Revision as of 16:24, 18 December 2007
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RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY
OverviewOverview
Here we present a time-resolved crystallographic analysis of the, hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1., The use of a slow substrate and fast crystallization methods made it, possible to perform the study with conventional data-collection, techniques. The results support the idea that the hydrolysis reaction, proceeds through a mechanism that is the inverse of the, transesterification reaction. In addition, the structures provide an, explanation for the differential behavior of RNase T1 towards exo- and, endo-cyclic thiophosphates.
About this StructureAbout this Structure
1GSP is a Single protein structure of sequence from Aspergillus oryzae with CA and SGP as ligands. Active as Ribonuclease T(1), with EC number 3.1.27.3 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
ReferenceReference
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218
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