Glyceraldehyde-3-Phosphate Dehydrogenase: Difference between revisions

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Nathan Line, David Canner, Andrew Swart, Alice Harmon, Michal Harel, Alexander Berchansky

Revision as of 01:54, 28 March 2010 view source Nathan Line (talk \| contribs) 75 edits No edit summary ← Older edit		Revision as of 02:14, 28 March 2010 view source Nathan Line (talk \| contribs) 75 edits No edit summary Newer edit →
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	The mechanism of the glycolysis reaction is fairly straight forward. After the aldehyde enters the <scene name='Nathan_Line_sandbox_3/Active_site/2'>active site</scene> (highlighted in green), the sulfhydryl group from <scene name='Nathan_Line_sandbox_3/Cystine/5'>Cystine 151</scene> attacks the nucleophilic carbon to form a thiohemiacetal. This intermediate undergoes oxidation due to a hydride transfer to a nearby NAD+ forming a thioester. From here, a phosphate group enters and attacks the same carbonyl while at the same time it is separated from the cystine by the protonated <scene name='Nathan_Line_sandbox_3/Histidine/2'>Histidine ~~176~~</scene> group. This produces the desired 1,3-bisphosphoglycerate.		The mechanism of the glycolysis reaction is fairly straight forward. After the aldehyde enters the <scene name='Nathan_Line_sandbox_3/Active_site/2'>active site</scene> (highlighted in green), the sulfhydryl group from <scene name='Nathan_Line_sandbox_3/Cystine/5'>Cystine 151</scene> attacks the nucleophilic carbon to form a thiohemiacetal. This intermediate undergoes oxidation due to a hydride transfer to a nearby NAD+ forming a thioester. From here, a phosphate group enters and attacks the same carbonyl while at the same time it is separated from the cystine by the protonated <scene name='Nathan_Line_sandbox_3/Histidine/3'>Histidine 178</scene> group. This produces the desired 1,3-bisphosphoglycerate.