1flo: Difference between revisions
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[[Image:1flo.gif|left|200px]]<br /> | [[Image:1flo.gif|left|200px]]<br /><applet load="1flo" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1flo" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1flo, resolution 2.65Å" /> | caption="1flo, resolution 2.65Å" /> | ||
'''FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I'''<br /> | '''FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1FLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with PHS as [http://en.wikipedia.org/wiki/ligand ligand]. | 1FLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with PHS as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=CTA:Active Catalytic Site Of Monomer A. Y343 Is Donated By M ...'>CTA</scene>, <scene name='pdbsite=CTB:Active Catalytic Site Of Monomer B. Y343 Is Donated By M ...'>CTB</scene>, <scene name='pdbsite=CTC:Inactive Catalytic Site Of Monomer C. Y343 Is Donated By ...'>CTC</scene>, <scene name='pdbsite=CTD:Inactive Catalytic Site Of Monomer D. Y343 Is Donated By ...'>CTD</scene> and <scene name='pdbsite=PHO:Scissle Phosphates'>PHO</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FLO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: tyrosine recombinase]] | [[Category: tyrosine recombinase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:16:24 2007'' | ||
Revision as of 16:06, 18 December 2007
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FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I
OverviewOverview
The crystal structure of a Flp recombinase tetramer bound to a Holliday, junction intermediate has been determined at 2.65 A resolution. Only one, of Flp's two domains, containing the active site, is structurally related, to other lambda integrase family site-specific recombinases, such as Cre., The Flp active site differs, however, in that the helix containing the, nucleophilic tyrosine is domain swapped, such that it cuts its DNA target, in trans. The Flp tetramer displays pseudo four-fold symmetry matching, that of the square planar Holliday junction substrate. This tetramer is, stabilized by additional novel trans interactions among monomers. The, structure illustrates how mechanistic unity is maintained on a chemical, level while allowing for substantial variation on the structural level, within a family of enzymes.
About this StructureAbout this Structure
1FLO is a Single protein structure of sequence from Saccharomyces cerevisiae with PHS as ligand. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping., Chen Y, Narendra U, Iype LE, Cox MM, Rice PA, Mol Cell. 2000 Oct;6(4):885-97. PMID:11090626
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