1e9z: Difference between revisions
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[[Image:1e9z.gif|left|200px]]<br /> | [[Image:1e9z.gif|left|200px]]<br /><applet load="1e9z" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1e9z" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1e9z, resolution 3.0Å" /> | caption="1e9z, resolution 3.0Å" /> | ||
'''CRYSTAL STRUCTURE OF HELICOBACTER PYLORI UREASE'''<br /> | '''CRYSTAL STRUCTURE OF HELICOBACTER PYLORI UREASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1E9Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] | 1E9Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] Known structural/functional Site: <scene name='pdbsite=NI1:Ni Binding Site For Residue B2002'>NI1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9Z OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: urease]] | [[Category: urease]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:07:29 2007'' | ||
Revision as of 15:57, 18 December 2007
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CRYSTAL STRUCTURE OF HELICOBACTER PYLORI UREASE
OverviewOverview
Helicobacter pylori, an etiologic agent in a variety of gastroduodenal, diseases, produces a large amount of urease, which is believed to, neutralize gastric acid by producing ammonia for the survival of the, bacteria. Up to 30% of the enzyme associates with the surface of intact, cells upon lysis of neighboring bacteria. The role of the enzyme at the, extracellular location has been a subject of controversy because the, purified enzyme is irreversibly inactivated below pH 5. We have determined, the crystal structure of H. pylori urease, which has a 1.1 MDa spherical, assembly of 12 catalytic units with an outer diameter of approximately 160, A. Under physiologically relevant conditions, the activity of the enzyme, remains unaffected down to pH 3. Activity assays under different, conditions indicated that the cluster of the 12 active sites on the, supramolecular assembly may be critical for the survival of the enzyme at, low pH. The structure provides a novel example of a molecular assembly, adapted for acid resistance that, together with the low Km value of the, enzyme, is likely to enable the organism to inhabit the hostile niche.
About this StructureAbout this Structure
1E9Z is a Protein complex structure of sequences from Helicobacter pylori with NI as ligand. Active as Urease, with EC number 3.5.1.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Supramolecular assembly and acid resistance of Helicobacter pylori urease., Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH, Nat Struct Biol. 2001 Jun;8(6):505-9. PMID:11373617
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