1bhl: Difference between revisions
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[[Image:1bhl.gif|left|200px]]<br /> | [[Image:1bhl.gif|left|200px]]<br /><applet load="1bhl" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1bhl" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1bhl, resolution 2.20Å" /> | caption="1bhl, resolution 2.20Å" /> | ||
'''CACODYLATED CATALYTIC DOMAIN OF HIV-1 INTEGRASE'''<br /> | '''CACODYLATED CATALYTIC DOMAIN OF HIV-1 INTEGRASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1BHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | 1BHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Known structural/functional Site: <scene name='pdbsite=ACT:Active Site'>ACT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BHL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: polyprotein]] | [[Category: polyprotein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:26:53 2007'' | ||
Revision as of 15:17, 18 December 2007
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CACODYLATED CATALYTIC DOMAIN OF HIV-1 INTEGRASE
OverviewOverview
Human immunodeficiency virus (HIV) integrase is the enzyme responsible for, insertion of a DNA copy of the viral genome into host DNA, an essential, step in the replication cycle of HIV. HIV-1 integrase comprises three, functional and structural domains: an N-terminal zinc-binding domain, a, catalytic core domain and a C-terminal DNA-binding domain. The catalytic, core domain with the F185H mutation has been crystallized without sodium, cacodylate in a new crystal form, free and complexed with the catalytic, metal Mg2+. The structures have been determined and refined to about 2.2, A. Unlike the previously reported structures, the three active-site, carboxylate residues (D,D-35-E motif) are well ordered and both aspartate, residues delineate a proper metal-binding site. Comparison of the active, binding site of this domain with that of other members from the, polynucleotidyl transferases superfamily shows a high level of similarity, providing a confident template for the design of antiviral agents.
About this StructureAbout this Structure
1BHL is a Single protein structure of sequence from Human immunodeficiency virus 1. Active as RNA-directed DNA polymerase, with EC number 2.7.7.49 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of the catalytic domain of HIV-1 integrase free and complexed with its metal cofactor: high level of similarity of the active site with other viral integrases., Maignan S, Guilloteau JP, Zhou-Liu Q, Clement-Mella C, Mikol V, J Mol Biol. 1998 Sep 18;282(2):359-68. PMID:9735293
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