1aox: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1aox.gif|left|200px]]<br /> | [[Image:1aox.gif|left|200px]]<br /><applet load="1aox" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1aox" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1aox, resolution 2.1Å" /> | caption="1aox, resolution 2.1Å" /> | ||
'''I DOMAIN FROM INTEGRIN ALPHA2-BETA1'''<br /> | '''I DOMAIN FROM INTEGRIN ALPHA2-BETA1'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
1AOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. | 1AOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=MGA:Mg Binding Site'>MGA</scene> and <scene name='pdbsite=MGB:Mg Binding Site'>MGB</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AOX OCA]. | ||
==Reference== | ==Reference== | ||
| Line 26: | Line 25: | ||
[[Category: integrin]] | [[Category: integrin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:19:14 2007'' | ||
Revision as of 15:09, 18 December 2007
|
I DOMAIN FROM INTEGRIN ALPHA2-BETA1
OverviewOverview
We have determined the high resolution crystal structure of the I domain, from the alpha-subunit of the integrin alpha2beta1, a cell surface, adhesion receptor for collagen and the human pathogen echovirus-1. The, domain, as expected, adopts the dinucleotide-binding fold, and contains a, metal ion-dependent adhesion site motif with bound Mg2+ at the top of the, beta-sheet. Comparison with the crystal structures of the leukocyte, integrin I domains reveals a new helix (the C-helix) protruding from the, metal ion-dependent adhesion site face of the domain which creates a, groove centered on the magnesium ion. Modeling of a collagen triple helix, into the groove suggests that a glutamic acid side chain from collagen can, coordinate the metal ion, and that the C-helix insert is a major, determinant of binding specificity. The binding site for echovirus-1 maps, to a distinct surface of the alpha2-I domain (one edge of the beta-sheet), consistent with data showing that virus and collagen binding occur by, different mechanisms. Comparison with the homologous von Willebrand factor, A3 domain, which also binds collagen, suggests that the two domains bind, collagen in different ways.
DiseaseDisease
Known diseases associated with this structure: Glycoprotein Ia deficiency (1) OMIM:[192974], Neonatal alloimmune thrombocytopenia OMIM:[192974]
About this StructureAbout this Structure
1AOX is a Single protein structure of sequence from Homo sapiens with MG as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the I domain from integrin alpha2beta1., Emsley J, King SL, Bergelson JM, Liddington RC, J Biol Chem. 1997 Nov 7;272(45):28512-7. PMID:9353312
Page seeded by OCA on Tue Dec 18 14:19:14 2007