1wc1: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
1WC1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 1WC1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arthrospira_platensis Arthrospira platensis]] with MG and TAT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WC1 OCA]]. | ||
==Reference== | ==Reference== | ||
Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15619637 15619637] | Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15619637 15619637] | ||
[[Category: Adenylate cyclase]] | |||
[[Category: Arthrospira platensis]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Buck, J.]] | [[Category: Buck, J.]] | ||
[[Category: Levin, L.R.]] | [[Category: Levin, L.R.]] | ||
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[[Category: soluble adenylyl cyclase]] | [[Category: soluble adenylyl cyclase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:20:03 2007'' | ||
Revision as of 11:15, 30 October 2007
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SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH RP-ATPALPHAS
OverviewOverview
In an evolutionarily conserved signaling pathway, 'soluble' adenylyl, cyclases (sACs) synthesize the ubiquitous second messenger cyclic, adenosine 3',5'-monophosphate (cAMP) in response to bicarbonate and, calcium signals. Here, we present crystal structures of a cyanobacterial, sAC enzyme in complex with ATP analogs, calcium and bicarbonate, which, represent distinct catalytic states of the enzyme. The structures reveal, that calcium occupies the first ion-binding site and directly mediates, nucleotide binding. The single ion-occupied, nucleotide-bound state, defines a novel, open adenylyl cyclase state. In contrast, bicarbonate, increases the catalytic rate by inducing marked active site closure and, recruiting a second, catalytic ion. The phosphates of the bound substrate, analogs are ... [(full description)]
About this StructureAbout this Structure
1WC1 is a [Single protein] structure of sequence from [Arthrospira platensis] with MG and TAT as [ligands]. Active as [Adenylate cyclase], with EC number [4.6.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:15619637
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