1wcv: Difference between revisions
No edit summary |
No edit summary |
||
| Line 24: | Line 24: | ||
[[Category: soj]] | [[Category: soj]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:19:22 2007'' | ||
Revision as of 11:14, 30 October 2007
|
STRUCTURE OF THE BACTERIAL CHROMOSOME SEGREGATION PROTEIN SOJ
OverviewOverview
Soj and Spo0J of the Gram-negative hyperthermophile Thermus thermophilus, belong to the conserved ParAB family of bacterial proteins implicated in, plasmid and chromosome partitioning. Spo0J binds to DNA near the, replication origin and localises at the poles following initiation of, replication. Soj oscillates in the nucleoid region in an ATP- and, Spo0J-dependent fashion. Here, we show that Soj undergoes ATP-dependent, dimerisation in solution and forms nucleoprotein filaments with DNA., Crystal structures of Soj in three nucleotide states demonstrate that the, empty and ADP-bound states are monomeric, while a hydrolysis-deficient, mutant, D44A, is capable of forming a nucleotide 'sandwich' dimer. Soj, ATPase activity is stimulated by Spo0J or the N-terminal 20 amino-acid, peptide of ... [(full description)]
About this StructureAbout this Structure
1WCV is a [Single protein] structure of sequence from [Thermus thermophilus] with CL and IOD as [ligands]. Structure known Active Site: 1. Full crystallographic information is available from [OCA].
ReferenceReference
Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch., Leonard TA, Butler PJ, Lowe J, EMBO J. 2005 Jan 26;24(2):270-82. Epub 2005 Jan 6. PMID:15635448
Page seeded by OCA on Tue Oct 30 10:19:22 2007