Fructose Bisphosphate Aldolase: Difference between revisions
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'''Fructose biphosphate aldolase''' | '''Fructose biphosphate aldolase''' | ||
Fructose biphosphate aldolase is an enzyme in glycolysis. It catalyzes the breakdown of fructose-1,6-biophosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-2-phosphate (GAP). The reaction is an aldol cleavage, or otherwise termed, retro aldo condensation. Catalysis occurs by the formation of a Schiff base (an imine resulting from a ketone and amine) from the amine of the aldolase's Lys229 and the open-ring form of FBP accompanied by stabilization from Asp33. <scene name='Austin_Drake_Sandbox/Catalytic_site/1'>Catalytic Site</scene> <ref name="jmol">Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/<ref/> Aldol cleavage produces GAP and an enamine precursor to DHAP. Tautomerization, protonation and the hydrolysis of the Schiff base produce the final product of DHAP and the active enzyme.<ref>Voet, D, Voet, J, & Pratt, C. (2008). Fundamentals of biochemistry, third edition. Hoboken, NJ: Wiley & Sons, Inc.<ref/> | Fructose biphosphate aldolase is an enzyme in glycolysis. It catalyzes the breakdown of fructose-1,6-biophosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-2-phosphate (GAP). The reaction is an aldol cleavage, or otherwise termed, retro aldo condensation. Catalysis occurs by the formation of a Schiff base (an imine resulting from a ketone and amine) from the amine of the aldolase's Lys229 and the open-ring form of FBP accompanied by stabilization from Asp33. <scene name='Austin_Drake_Sandbox/Catalytic_site/1'>Catalytic Site</scene> <ref name="jmol"> Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/<ref/> Aldol cleavage produces GAP and an enamine precursor to DHAP. Tautomerization, protonation and the hydrolysis of the Schiff base produce the final product of DHAP and the active enzyme.<ref>Voet, D, Voet, J, & Pratt, C. (2008). Fundamentals of biochemistry, third edition. Hoboken, NJ: Wiley & Sons, Inc.<ref/> | ||
<scene name='Austin_Drake_Sandbox/B_sheet_barrel/2'>FBP fits here</scene><refname="jmol" /> | <scene name='Austin_Drake_Sandbox/B_sheet_barrel/2'>FBP fits here</scene><refname="jmol" /> | ||
Revision as of 05:15, 26 February 2010
Fructose biphosphate aldolase
Fructose biphosphate aldolase is an enzyme in glycolysis. It catalyzes the breakdown of fructose-1,6-biophosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-2-phosphate (GAP). The reaction is an aldol cleavage, or otherwise termed, retro aldo condensation. Catalysis occurs by the formation of a Schiff base (an imine resulting from a ketone and amine) from the amine of the aldolase's Lys229 and the open-ring form of FBP accompanied by stabilization from Asp33. <ref name="jmol"> Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/Cite error: The opening <ref> tag is malformed or has a bad name Aldol cleavage produces GAP and an enamine precursor to DHAP. Tautomerization, protonation and the hydrolysis of the Schiff base produce the final product of DHAP and the active enzyme.<ref>Voet, D, Voet, J, & Pratt, C. (2008). Fundamentals of biochemistry, third edition. Hoboken, NJ: Wiley & Sons, Inc.Cite error: The opening <ref> tag is malformed or has a bad name
<refname="jmol" /> Template:STRUCTURE 2ald