2bog: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2BOG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 2BOG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BOG OCA]]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16185060 16185060] | Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16185060 16185060] | ||
[[Category: Cellulase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: | [[Category: Thermobifida fusca]] | ||
[[Category: Bergfors, T.]] | [[Category: Bergfors, T.]] | ||
[[Category: Driguez, H.]] | [[Category: Driguez, H.]] | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:07:13 2007'' | ||
Revision as of 11:02, 30 October 2007
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CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE
OverviewOverview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4, linkages in cellulose at accessible points along the polymer. The, structure of the catalytic domain of Cel6A from T. fusca in complex with a, nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been, determined to 1.5 A resolution. The glycosyl unit in subsite -1 was, sterically hindered by Tyr73 and forced into a distorted (2)S(o), conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a, relaxed (4)C(1) chair conformation. The relaxed conformation was seen in, two complex structures of the mutated enzyme, with cellotetrose (Glc(4)), at 1.64 A and ... [(full description)]
About this StructureAbout this Structure
2BOG is a [Single protein] structure of sequence from [Thermobifida fusca]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060
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