Proteopedia

Group:SMART:2010 Pingry SMART Team: Difference between revisions

← Older editNewer edit →
Caryn Ha (talk | contribs)
72 edits
Undo revision 1041932 by Caryn Ha (Talk)
Caryn Ha (talk | contribs)
72 edits
Undo revision 1041933 by Caryn Ha (Talk)
Line 20: Line 20:


'''Design description'''
'''Design description'''
The conformation of 2,5-DKGR (diketo-d-gluconic acid reductase)is a parallel alpha/beta barrel of <scene name='2010_Pingry_SMART_Team/1m9h_original/14'>eight alpha helices (highlighted red) and eight beta sheets (highlighted blue).</scene> Notably, the alpha/beta 8 barrel structure is demonstrated by other enzymes of the aldo-keto reductase family.
 
<scene name='2010_Pingry_SMART_Team/1m9h_original/16'>Mutations of 2.5-DKGR's conformation have been conducted to alternate the cofactor specificity to NADH rather than NADPH. Alternate cofactor (NADH)for mutant 2,5 DKGR shown in wireframe and colored CPK.</scene>


Mutations of the 2,5-DKGR's conformation have been conducted for alternate cofactor specificty to <scene name='2010_Pingry_SMART_Team/1m9h_original/16'>NADH(shown in wireframe and colored CPK)</scene> rather than NADPH.


The backbone of the four residues changed between WT and NADP-binding mutant are colored orange <scene name='2010_Pingry_SMART_Team/1m9h_original/17'>(Lys232Gly, Phe22Tyr, Arg238His, Ala272Gly).</scene>
The backbone of the four residues changed between WT and NADP-binding mutant are colored orange <scene name='2010_Pingry_SMART_Team/1m9h_original/17'>(Lys232Gly, Phe22Tyr, Arg238His, Ala272Gly).</scene>
Retrieved from "https://proteopedia.openfox.io/w/Group:SMART:2010_Pingry_SMART_Team"