Group:SMART:2010 Pingry SMART Team: Difference between revisions
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==='''Inherent dual cofactor use, Xylose reductase'''=== | ==='''Inherent dual cofactor use, Xylose reductase'''=== | ||
- | -Xylose reductase is an unusual protein from the aldo-keto reductase superfamily in that the wild type is able to efficiently utilize both NADH and NADPH in its reduction of the 5 carbon sugar xylose into xylitol. Normally found in the yeast ''Candida tenuis'', it functions biologically as a homodimer unlike the majority of AKR proteins. While Dr. Banta is not actively researching this protein, Xylose Reductase's dual substrate specificity has influenced his engineering of AdhD. Because of its ability to change the conformation of two major loops, which enable different side chain conformations, Xylose Reductase can accomodate both the presence and absence of a phosphate in the cofactor.- | ||
<applet load='1K8C_chainD.pdb' size='400' frame='true' align='left' caption='1k8c, Xylose reductase with NADP+' scene='2010_Pingry_SMART_Team/1k8c_default/2'/> | <applet load='1K8C_chainD.pdb' size='400' frame='true' align='left' caption='1k8c, Xylose reductase with NADP+' scene='2010_Pingry_SMART_Team/1k8c_default/2'/> | ||
====PDB ID: 1k8c, Xylose reductase with NADP+==== | ====PDB ID: 1k8c, Xylose reductase with NADP+==== | ||