Group:SMART:2010 Pingry SMART Team: Difference between revisions

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== 2010 Pingry S.M.A.R.T. Team, Protein Engineering; AKR's for Biofuel Cells ==

[[Image:2010PingrySMARTTeam.jpg|frame|~~left~~|alt=alt text|(Left to Right) '''Front Row:''' Connie Wang, Edd Kong, Ed Xiao, Flo Ma, Caryn Ha, Mai-Lee Picard; '''Back Row:''' 2010 S.M.A.R.T. Team Advisor Tommie Hata, 2010 S.M.A.R.T. Team Mentor Scott Banta, Doug Ober, David Sukhin, Dylan Sun, Ricardo Vollbrechthausen, Graduate Student Elliot Campbell]]The 2010 [http://www.pingry.org Pingry School] S.M.A.R.T. Team (Students Modeling A Research Topic) is working with Dr. [http://www.cheme.columbia.edu/fac-bios/banta/faculty.html Scott Banta] and graduate student Elliot Campbell at Columbia University to learn about enzymes being engineered for use in biofuel cells. Features being engineered into these enzymes include (1) self-assembly into hydrogels, (2) alternate cofactor use, and (3) broader substrate specificity. AdhD alcohol dehydrogenase from the thermophile [http://en.wikipedia.org/wiki/Pyrococcus_furiosus ''Pyrococcus furiosus''] is one of the enzymes being engineered with these features by the Banta Lab. AdhD is a member of the aldo-keto reductase (AKR) family of oxidoreductases. Taking advantage of its innate thermostable properties, the Banta Lab is engineering AdhD for use in biofuel cells.

[[Image:2010PingrySMARTTeam.jpg|frame|right|alt=alt text|(Left to Right) '''Front Row:''' Connie Wang, Edd Kong, Ed Xiao, Flo Ma, Caryn Ha, Mai-Lee Picard; '''Back Row:''' 2010 S.M.A.R.T. Team Advisor Tommie Hata, 2010 S.M.A.R.T. Team Mentor Scott Banta, Doug Ober, David Sukhin, Dylan Sun, Ricardo Vollbrechthausen, Graduate Student Elliot Campbell]]The 2010 [http://www.pingry.org Pingry School] S.M.A.R.T. Team (Students Modeling A Research Topic) is working with Dr. [http://www.cheme.columbia.edu/fac-bios/banta/faculty.html Scott Banta] and graduate student Elliot Campbell at Columbia University to learn about enzymes being engineered for use in biofuel cells. Features being engineered into these enzymes include (1) self-assembly into hydrogels, (2) alternate cofactor use, and (3) broader substrate specificity. AdhD alcohol dehydrogenase from the thermophile [http://en.wikipedia.org/wiki/Pyrococcus_furiosus ''Pyrococcus furiosus''] is one of the enzymes being engineered with these features by the Banta Lab. AdhD is a member of the aldo-keto reductase (AKR) family of oxidoreductases. Taking advantage of its innate thermostable properties, the Banta Lab is engineering AdhD for use in biofuel cells.

The logical design and engineering of AdhD is based partially on the solved structures of other enzymes belonging to the AKR family of enzymes. Structures of mutants that bind alternate cofactors and those bound to its substrate provide insight into how to engineer AdhD and other enzymes of use in a biofuel cell. The 2010 Pingry S.M.A.R.T. Team is producing physical models of various AKR's that highlight the enzymes' structural and functional characteristics that are relevant to the Banta Lab's work.

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 == 2010 Pingry S.M.A.R.T. Team, Protein Engineering; AKR's for Biofuel Cells ==
-[[Image:2010PingrySMARTTeam.jpg|frame|left|alt=alt text|(Left to Right) '''Front Row:''' Connie Wang, Edd Kong, Ed Xiao, Flo Ma, Caryn Ha, Mai-Lee Picard; '''Back Row:''' 2010 S.M.A.R.T. Team Advisor Tommie Hata, 2010 S.M.A.R.T. Team Mentor Scott Banta, Doug Ober, David Sukhin, Dylan Sun, Ricardo Vollbrechthausen, Graduate Student Elliot Campbell]]The 2010 [http://www.pingry.org Pingry School] S.M.A.R.T. Team (Students Modeling A Research Topic) is working with Dr. [http://www.cheme.columbia.edu/fac-bios/banta/faculty.html Scott Banta] and graduate student Elliot Campbell at Columbia University to learn about enzymes being engineered for use in biofuel cells.  Features being engineered into these enzymes include (1) self-assembly into hydrogels, (2) alternate cofactor use, and (3) broader substrate specificity.  AdhD alcohol dehydrogenase from the thermophile [http://en.wikipedia.org/wiki/Pyrococcus_furiosus ''Pyrococcus furiosus''] is one of the enzymes being engineered with these features by the Banta Lab.  AdhD is a member of the aldo-keto reductase (AKR) family of oxidoreductases.  Taking advantage of its innate thermostable properties, the Banta Lab is engineering AdhD for use in biofuel cells.
+[[Image:2010PingrySMARTTeam.jpg|frame|right|alt=alt text|(Left to Right) '''Front Row:''' Connie Wang, Edd Kong, Ed Xiao, Flo Ma, Caryn Ha, Mai-Lee Picard; '''Back Row:''' 2010 S.M.A.R.T. Team Advisor Tommie Hata, 2010 S.M.A.R.T. Team Mentor Scott Banta, Doug Ober, David Sukhin, Dylan Sun, Ricardo Vollbrechthausen, Graduate Student Elliot Campbell]]The 2010 [http://www.pingry.org Pingry School] S.M.A.R.T. Team (Students Modeling A Research Topic) is working with Dr. [http://www.cheme.columbia.edu/fac-bios/banta/faculty.html Scott Banta] and graduate student Elliot Campbell at Columbia University to learn about enzymes being engineered for use in biofuel cells.  Features being engineered into these enzymes include (1) self-assembly into hydrogels, (2) alternate cofactor use, and (3) broader substrate specificity.  AdhD alcohol dehydrogenase from the thermophile [http://en.wikipedia.org/wiki/Pyrococcus_furiosus ''Pyrococcus furiosus''] is one of the enzymes being engineered with these features by the Banta Lab.  AdhD is a member of the aldo-keto reductase (AKR) family of oxidoreductases.  Taking advantage of its innate thermostable properties, the Banta Lab is engineering AdhD for use in biofuel cells.
 The logical design and engineering of AdhD is based partially on the solved structures of other enzymes belonging to the AKR family of enzymes.  Structures of mutants that bind alternate cofactors and those bound to its substrate provide insight into how to engineer AdhD and other enzymes of use in a biofuel cell.  The 2010 Pingry S.M.A.R.T. Team is producing physical models of various AKR's that highlight the enzymes' structural and functional characteristics that are relevant to the Banta Lab's work.