1a6i: Difference between revisions
New page: left|200px<br /> <applet load="1a6i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a6i, resolution 2.4Å" /> '''TET REPRESSOR, CLASS... |
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==About this Structure== | ==About this Structure== | ||
1A6I is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A6I OCA]]. | 1A6I is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Structure known Active Site: TNE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A6I OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:48:23 2007'' | ||
Revision as of 09:43, 30 October 2007
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TET REPRESSOR, CLASS D VARIANT
OverviewOverview
The X-ray crystal structure analysis of inducer-free Tet repressor, TetR, at 2.4 A resolution identifies one of two openings of the tunnel-like, binding site as the entrance for the inducer tetracycline-Mg2+, [Mg Tc]+., Recognition and binding of the inducer unleashes conformational changes, leading to the induced state of TetR. In the first step, the C-terminal, turn of alpha-helix 6 unwinds, thereby altering the orientation of, alpha-helix 4. This different orientation of alpha-helix 4 is stabilized, by a series of hydrogen bonds mediated through a chain of eight water, molecules. The alpha-helix 4 connects the DNA-binding domain, (alpha-helices 1 to 3) to the rigid TetR core, and thus regulates gene, expression through its respective orientations.
About this StructureAbout this Structure
1A6I is a [Single protein] structure of sequence from [Escherichia coli]. Structure known Active Site: TNE. Full crystallographic information is available from [OCA].
ReferenceReference
Conformational changes of the Tet repressor induced by tetracycline trapping., Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W, J Mol Biol. 1998 Jun 5;279(2):439-47. PMID:9642048
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