2c22: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2C22 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]] with CA and DGT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C22 OCA]]. | 2C22 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]] with CA and DGT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C22 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: translesion dna polymerase]] | [[Category: translesion dna polymerase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:48:10 2007'' | ||
Revision as of 09:43, 30 October 2007
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EFFICIENT AND HIGH FIDELITY INCORPORATION OF DCTP OPPOSITE 7,8-DIHYDRO-8-OXODEOXYGUANOSINE BY SULFOLOBUS SOLFATARICUS DNA POLYMERASE DPO4
OverviewOverview
DNA polymerases insert dATP opposite the oxidative damage product, 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) instead of dCTP, to the extent of, >90% with some polymerases. Steady-state kinetics with the Y-family, Sulfolobus solfataricus DNA polymerase IV (Dpo4) showed 90-fold higher, incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher, efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The, catalytic efficiency for these events (with dCTP or C) was similar for G, and 8-oxoG templates. Mass spectral analysis of extended DNA primers, showed >/=95% incorporation of dCTP > dATP opposite 8-oxoG., Pre-steady-state kinetics showed faster rates of dCTP incorporation, opposite 8-oxoG than G. The measured K(d)(,dCTP) was 15-fold lower for an, ... [(full description)]
About this StructureAbout this Structure
2C22 is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with CA and DGT as [ligands]. Active as [Transferase], with EC number [2.7.7.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Efficient and high fidelity incorporation of dCTP opposite 7,8-dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA polymerase Dpo4., Zang H, Irimia A, Choi JY, Angel KC, Loukachevitch LV, Egli M, Guengerich FP, J Biol Chem. 2006 Jan 27;281(4):2358-72. Epub 2005 Nov 22. PMID:16306039
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OCA- Pages with broken file links
- Single protein
- Sulfolobus solfataricus
- Egli, M.
- Irimia, A.
- Loukachevitch, L.V.
- CA
- DGT
- 7
- 8-dihydro-8-oxodeoxyguanosine
- Dna damage
- Dna repair
- Dna replication
- Dna-binding
- Dna-directed dna polymerase
- Magnesium
- Metal -binding
- Mutator protein
- Nucleotidyltransferase
- P2 dna polymerase iv
- Transferase
- Translesion dna polymerase