DNA Polymerase I: Difference between revisions
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==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ||
<applet load='1taq.pdb' size='450' frame='true' align='right' caption='Thermus aquaticus Pol I' scene= 'Sandbox_dvoet/DNA_polymerase/Taq_pol_i/1'/> | <applet load='1taq.pdb' size='450' frame='true' align='right' caption='Thermus aquaticus Pol I ([[1taq]])' scene= 'Sandbox_dvoet/DNA_polymerase/Taq_pol_i/1'/> | ||
As mentioned above, Pol I's primary and essential function is to excise the RNA primers from newly synthesized Okazaki fragments with its 5' → 3' exonuclease function and replace them with DNA using its polymerase function. This yields a double-stranded DNA (dsDNA) with a single strand nick between successive Okazaki fragments, a nick that is eventually sealed through the action of DNA ligase. | As mentioned above, Pol I's primary and essential function is to excise the RNA primers from newly synthesized Okazaki fragments with its 5' → 3' exonuclease function and replace them with DNA using its polymerase function. This yields a double-stranded DNA (dsDNA) with a single strand nick between successive Okazaki fragments, a nick that is eventually sealed through the action of DNA ligase. | ||
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{{Clear}} | {{Clear}} | ||
==Structures of Klentaq1 in its closed and open forms== | ==Structures of Klentaq1 in its closed and open forms== | ||