1hkc: Difference between revisions
New page: left|200px<br /> <applet load="1hkc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hkc, resolution 2.80Å" /> '''RECOMBINANT HUMAN H... |
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==About this Structure== | ==About this Structure== | ||
1HKC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with GLC, PO4 and K as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKC OCA]]. | 1HKC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with GLC, PO4 and K as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1]]. Structure known Active Sites: GLN, MEC, MEN, PIC and PIN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKC OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
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Revision as of 09:41, 30 October 2007
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RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE
OverviewOverview
Hexokinase I, the pacemaker of glycolysis in brain tissue and red blood, cells, is comprised of two similar domains fused into a single polypeptide, chain. The C-terminal half of hexokinase I is catalytically active, whereas the N-terminal half is necessary for the relief of product, inhibition by phosphate. A crystalline complex of recombinant human, hexokinase I with glucose and phosphate (2.8 A resolution) reveals a, single binding site for phosphate and glucose at the N-terminal half of, the enzyme. Glucose and phosphate stabilize the N-terminal half in a, closed conformation. Unexpectedly, glucose binds weakly to the C-terminal, half of the enzyme and does not by itself stabilize a closed conformation., Evidently a stable, closed C-terminal half requires either ATP or glucose, ... [(full description)]
About this StructureAbout this Structure
1HKC is a [Single protein] structure of sequence from [Homo sapiens] with GLC, PO4 and K as [ligands]. Active as [Transferase], with EC number [2.7.1.1]. Structure known Active Sites: GLN, MEC, MEN, PIC and PIN. Full crystallographic information is available from [OCA].
ReferenceReference
Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:9735292
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