Polyneuridine Aldehyde Esterase: Difference between revisions
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Up to now the phytochemical analysis of ''Rauvolfia'' in vitro systems resulted in the isolation of more than 30 monoterpenoid indole alkaloids. Many of them belong to the well known ajmalan-sarpagan type alkaloids. One of the most intriguing alkaloid was '''ajmaline''', a compound comprising a complex hexacyclic ring system bearing nine chiral carbon centers. | Up to now the phytochemical analysis of ''Rauvolfia'' in vitro systems resulted in the isolation of more than 30 monoterpenoid indole alkaloids. Many of them belong to the well known ajmalan-sarpagan type alkaloids. One of the most intriguing alkaloid was '''ajmaline''', a compound comprising a complex hexacyclic ring system bearing nine chiral carbon centers. | ||
'''Polyneuridine Aldehyde Esterase''''''(PNAE)''' is an important enzyme during Ajmaline Biosynthesis, transforming C-10 skeleton polyneuridine aldehyde into C-9 skeleton epi-vellosimine.[[Image: | '''Polyneuridine Aldehyde Esterase''''''(PNAE)''' is an important enzyme during Ajmaline Biosynthesis, transforming C-10 skeleton polyneuridine aldehyde into C-9 skeleton epi-vellosimine.[[Image:Pathway.JPG]] | ||
For the biochemical characterization of '''PNAE''', the enzyme was isolated from plant cell cultures of the Indian medicinal plant ''Rauvolfia serpentina'' and partially sequenced; the '''PNAE''' cDNA was then cloned and overexpressed in ''Escherichia coli''. | For the biochemical characterization of '''PNAE''', the enzyme was isolated from plant cell cultures of the Indian medicinal plant ''Rauvolfia serpentina'' and partially sequenced; the '''PNAE''' cDNA was then cloned and overexpressed in ''Escherichia coli''. | ||