User:Wayne Decatur/3fpn Morph methods: Difference between revisions

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Wayne Decatur (talk | contribs)
Wayne Decatur (talk | contribs)
Line 39: Line 39:
Then draw measurements between using menu
Then draw measurements between using menu


select 173:a and sidechain and 1.8; wireframe 0.55; color cpk;
select 173:a and 1.8; wireframe 0.55; color cpk;
select 196:b and sidechain and 1.8; wireframe 0.55;color cpk;
select 196:b and sidechain and 1.8; wireframe 0.55;color cpk;


Line 70: Line 70:
select 185:a and sidechain; wireframe 0.55; color cpk;
select 185:a and sidechain; wireframe 0.55; color cpk;
select 223:b and sidechain; wireframe 0.55;color cpk;
select 223:b and sidechain; wireframe 0.55;color cpk;
select 173:a and sidechain; wireframe 0.55; color cpk;
select 173:a; wireframe 0.55; color cpk;
select 196:b and sidechain; wireframe 0.55;color cpk;
select 196:b and sidechain; wireframe 0.55;color cpk;
select 176:a and sidechain; wireframe 0.55; color cpk;
select 176:a and sidechain; wireframe 0.55; color cpk;

Revision as of 10:50, 24 October 2009

Moving to match Figure 3Moving to match Figure 3

Using Pymol and the 3fpn file, I moved so interface is perpendicular to y axis:

translate [10,0,0], chain b

rotate y, 65, chain b

Saved molecule.

Morph from normal 3fpn structure to view in Figure 3 of article describing the structureMorph from normal 3fpn structure to view in Figure 3 of article describing the structure

Insert caption here

Drag the structure with the mouse to rotate

Took the two files and submitted them. Since the structures didn't have nucleic acids, I took the advice here and used the Yale Morph Server for morphing complexes.

Uploaded to Proteopedia File:3fpntorotatedversion.pdb.
loaded '3fpntorotatedversion.pdb' in Scene Authoring Tools.




[Control the animation with the 'animation' submenu on the menu that comes up if you click on the Jmol frank in the bottom rigth corner. Also, if the animation seems to be stuck, scroll in the bar on the right of your browser.]

Paper on the structurePaper on the structure

[xtra 1]

  1. Pakotiprapha D, Liu Y, Verdine GL, Jeruzalmi D. A structural model for the damage-sensing complex in bacterial nucleotide excision repair. J Biol Chem. 2009 May 8;284(19):12837-44. Epub 2009 Mar 13. PMID:19287003 doi:10.1074/jbc.M900571200