User:Wayne Decatur/3fpn Morph methods: Difference between revisions

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Wayne Decatur (talk | contribs)
New page: ==Moving to match Figure 3== Using Pymol and the 3fpn file, I moved so interface is perpendicular to y axis: translate [10,0,0], chain b rotate y, 65, chain b Saved molecule. ==Morph ...
 
Wayne Decatur (talk | contribs)
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select 219:a and sidechain and 1.8; wireframe 0.55; color cpk;
select 219:a and sidechain and 1.8; wireframe 0.55; color cpk;
select 183:b and sidechain and 1.8; wireframe 0.55;color cpk;
select 183:b and sidechain and 1.8; wireframe 0.55;color cpk;
Then draw measurements between using menu
select 170:a and sidechain and 1.8; wireframe 0.55; color cpk;
select 231:b and sidechain and 1.8; wireframe 0.55;color cpk;


Then draw measurements between using menu
Then draw measurements between using menu
Line 36: Line 42:
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<scene name='3fpn_Morph_methods/Testmeasureanim/1'>Test 1.8 wireframe and measures</scene>
<scene name='3fpn_Morph_methods/Testmeasureanim/2'>Test 1.8 wireframe and measures</scene>


==Paper on the structure==
==Paper on the structure==
<ref group="xtra">PMID:19287003</ref><references group="xtra"/>
<ref group="xtra">PMID:19287003</ref><references group="xtra"/>

Revision as of 07:39, 23 October 2009

Moving to match Figure 3Moving to match Figure 3

Using Pymol and the 3fpn file, I moved so interface is perpendicular to y axis:

translate [10,0,0], chain b

rotate y, 65, chain b

Saved molecule.

Morph from normal 3fpn structure to view in Figure 3 of article describing the structureMorph from normal 3fpn structure to view in Figure 3 of article describing the structure

Insert caption here

Drag the structure with the mouse to rotate

Took the two files and submitted them. Since the structures didn't have nucleic acids, I took the advice here and used the Yale Morph Server for morphing complexes.

Uploaded to Proteopedia File:3fpntorotatedversion.pdb.
loaded '3fpntorotatedversion.pdb'.


Paper on the structurePaper on the structure

[xtra 1]

  1. Pakotiprapha D, Liu Y, Verdine GL, Jeruzalmi D. A structural model for the damage-sensing complex in bacterial nucleotide excision repair. J Biol Chem. 2009 May 8;284(19):12837-44. Epub 2009 Mar 13. PMID:19287003 doi:10.1074/jbc.M900571200