1ha3: Difference between revisions
New page: left|200px<br /> <applet load="1ha3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ha3, resolution 2.00Å" /> '''ELONGATION FACTOR T... |
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==About this Structure== | ==About this Structure== | ||
1HA3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]] with MG, GDP, MAU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.5.1 3.1.5.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HA3 OCA]]. | 1HA3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]] with MG, GDP, MAU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.5.1 3.1.5.1]]. Structure known Active Sites: GDA, GDB, MAA, MAB, MGA and MGB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HA3 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: ribosome]] | [[Category: ribosome]] | ||
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Revision as of 09:37, 30 October 2007
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ELONGATION FACTOR TU IN COMPLEX WITH AURODOX
OverviewOverview
Aurodox is a member of the family of kirromycin antibiotics, which inhibit, protein biosynthesis by binding to elongation factor Tu (EF-Tu). We have, determined the crystal structure of the 1:1:1 complex of Thermus, thermophilus EF-Tu with GDP and aurodox to 2.0-A resolution. During its, catalytic cycle, EF-Tu adopts two strikingly different conformations, depending on the nucleotide bound: the GDP form and the GTP form. In the, present structure, a GTP complex-like conformation of EF-Tu is observed, although GDP is bound to the nucleotide-binding site. This is consistent, with previous proposals that aurodox fixes EF-Tu on the ribosome by, locking it in its GTP form. Binding of EF-Tu.GDP to aminoacyl-tRNA and, mutually exclusive binding of kirromycin and elongation factor Ts to EF-Tu, ... [(full description)]
About this StructureAbout this Structure
1HA3 is a [Single protein] structure of sequence from [Thermus aquaticus] with MG, GDP, MAU and BME as [ligands]. Active as [Hydrolase], with EC number [3.1.5.1]. Structure known Active Sites: GDA, GDB, MAA, MAB, MGA and MGB. Full crystallographic information is available from [OCA].
ReferenceReference
Conformational change of elongation factor Tu (EF-Tu) induced by antibiotic binding. Crystal structure of the complex between EF-Tu.GDP and aurodox., Vogeley L, Palm GJ, Mesters JR, Hilgenfeld R, J Biol Chem. 2001 May 18;276(20):17149-55. Epub 2001 Jan 30. PMID:11278992
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