1ecb: Difference between revisions
New page: left|200px<br /> <applet load="1ecb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ecb, resolution 2.7Å" /> '''ESCHERICHIA COLI GLU... |
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==About this Structure== | ==About this Structure== | ||
1ECB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG and 5GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECB OCA]]. | 1ECB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG and 5GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14]]. Structure known Active Sites: NTA, NTB, NTC, NTD, PRA, PRB, PRC and PRT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECB OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 09:33, 30 October 2007
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ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT
OverviewOverview
Activation of gluatmine phosphoribosylpyrophosphate (RPPP), amidotransferase (GPATase) by binding of a PRPP substrate analog results, in the formation of a 20 A channel connecting the active site for, glutamine hydrolysis in one domain with the PRPP site in a second domain., This solvent-inaccessible channel permits transfer of the NH3 intermediate, between the two active sites. Tunneling of NH3 may be a common mechanism, for glutamine amidotransferase-catalyzed nitrogen transfer and for, coordination of catalysis at two distinct active sites in complex enzymes., The 2.4 A crystal structure of the active conformer of GPATase also, provides the first description of an intact active site for the, phosphoribosyltransferase (PRTase) family of nucleotide synthesis and, salvage enzymes. ... [(full description)]
About this StructureAbout this Structure
1ECB is a [Single protein] structure of sequence from [Escherichia coli] with MG and 5GP as [ligands]. Active as [Transferase], with EC number [2.4.2.14]. Structure known Active Sites: NTA, NTB, NTC, NTD, PRA, PRB, PRC and PRT. Full crystallographic information is available from [OCA].
ReferenceReference
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site., Krahn JM, Kim JH, Burns MR, Parry RJ, Zalkin H, Smith JL, Biochemistry. 1997 Sep 16;36(37):11061-8. PMID:9333323
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