1oux: Difference between revisions

Revision as of 15:21, 21 February 2008

LecB (PA-LII) sugar-free

OverviewOverview

The crystal structure of Pseudomonas aeruginosa fucose-specific lectin LecB was determined in its metal-bound and metal-free state as well as in complex with fucose, mannose and fructopyranose. All three monosaccharides bind isosterically via direct interactions with two calcium ions as well as direct hydrogen bonds with several side-chains. The higher affinity for fucose is explained by the details of the binding site around C6 and O1 of fucose. In the mannose and fructose complexes, a carboxylate oxygen atom and one or two hydroxyl groups are partly shielded from solvent upon sugar binding, preventing them from completely fulfilling their hydrogen bonding potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon demetallization, the C terminus as well as the otherwise rigid metal-binding loop become more mobile and adopt multiple conformations.

About this StructureAbout this Structure

1OUX is a Single protein structure of sequence from Pseudomonas aeruginosa with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:12909014

Page seeded by OCA on Thu Feb 21 14:21:54 2008

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OCA

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 '''LecB (PA-LII) sugar-free'''<br />
 ==Overview==
-The crystal structure of Pseudomonas aeruginosa fucose-specific lectin, LecB was determined in its metal-bound and metal-free state as well as in, complex with fucose, mannose and fructopyranose. All three monosaccharides, bind isosterically via direct interactions with two calcium ions as well, as direct hydrogen bonds with several side-chains. The higher affinity for, fucose is explained by the details of the binding site around C6 and O1 of, fucose. In the mannose and fructose complexes, a carboxylate oxygen atom, and one or two hydroxyl groups are partly shielded from solvent upon sugar, binding, preventing them from completely fulfilling their hydrogen bonding, potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon, demetallization, the C terminus as well as the otherwise rigid, metal-binding loop become more mobile and adopt multiple conformations.
+The crystal structure of Pseudomonas aeruginosa fucose-specific lectin LecB was determined in its metal-bound and metal-free state as well as in complex with fucose, mannose and fructopyranose. All three monosaccharides bind isosterically via direct interactions with two calcium ions as well as direct hydrogen bonds with several side-chains. The higher affinity for fucose is explained by the details of the binding site around C6 and O1 of fucose. In the mannose and fructose complexes, a carboxylate oxygen atom and one or two hydroxyl groups are partly shielded from solvent upon sugar binding, preventing them from completely fulfilling their hydrogen bonding potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon demetallization, the C terminus as well as the otherwise rigid metal-binding loop become more mobile and adopt multiple conformations.
 ==About this Structure==
-OUX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CA and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OUX OCA].
+OUX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUX OCA].
 ==Reference==
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 [[Category: Pseudomonas aeruginosa]]
 [[Category: Single protein]]
-[[Category: Jaeger, K.E.]]
+[[Category: Jaeger, K E.]]
 [[Category: Loris, R.]]
 [[Category: Tielker, D.]]
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 [[Category: lectin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:29:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:54 2008''