1ka2: Difference between revisions

Revision as of 14:31, 21 February 2008

Structure of Pyrococcus furiosus Carboxypeptidase Apo-Mg

OverviewOverview

The structure of Pyrococcus furiosus carboxypeptidase (PfuCP) has been determined to 2.2 A resolution using multiwavelength anomalous diffraction (MAD) methods. PfuCP represents the first structure of the new M32 family of carboxypeptidases. The overall structure is comprised of a homodimer. Each subunit is mostly helical with its most pronounced feature being a deep substrate binding groove. The active site lies at the bottom of this groove and contains an HEXXH motif that coordinates the metal ion required for catalysis. Surprisingly, the structure is similar to the recently reported rat neurolysin. Comparison of these structures as well as sequence analyses with other homologous proteins reveal several conserved residues. The roles for these conserved residues in the catalytic mechanism are inferred based on modeling and their location.

About this StructureAbout this Structure

1KA2 is a Single protein structure of sequence from Pyrococcus furiosus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a novel carboxypeptidase from the hyperthermophilic archaeon Pyrococcus furiosus., Arndt JW, Hao B, Ramakrishnan V, Cheng T, Chan SI, Chan MK, Structure. 2002 Feb;10(2):215-24. PMID:11839307

Page seeded by OCA on Thu Feb 21 13:31:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1ka2.jpg|left|200px]]<br /><applet load="1ka2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ka2.jpg|left|200px]]<br /><applet load="1ka2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ka2, resolution 2.20&Aring;" />
 '''Structure of Pyrococcus furiosus Carboxypeptidase Apo-Mg'''<br />
 ==Overview==
-The structure of Pyrococcus furiosus carboxypeptidase (PfuCP) has been, determined to 2.2 A resolution using multiwavelength anomalous diffraction, (MAD) methods. PfuCP represents the first structure of the new M32 family, of carboxypeptidases. The overall structure is comprised of a homodimer., Each subunit is mostly helical with its most pronounced feature being a, deep substrate binding groove. The active site lies at the bottom of this, groove and contains an HEXXH motif that coordinates the metal ion required, for catalysis. Surprisingly, the structure is similar to the recently, reported rat neurolysin. Comparison of these structures as well as, sequence analyses with other homologous proteins reveal several conserved, residues. The roles for these conserved residues in the catalytic, mechanism are inferred based on modeling and their location.
+The structure of Pyrococcus furiosus carboxypeptidase (PfuCP) has been determined to 2.2 A resolution using multiwavelength anomalous diffraction (MAD) methods. PfuCP represents the first structure of the new M32 family of carboxypeptidases. The overall structure is comprised of a homodimer. Each subunit is mostly helical with its most pronounced feature being a deep substrate binding groove. The active site lies at the bottom of this groove and contains an HEXXH motif that coordinates the metal ion required for catalysis. Surprisingly, the structure is similar to the recently reported rat neurolysin. Comparison of these structures as well as sequence analyses with other homologous proteins reveal several conserved residues. The roles for these conserved residues in the catalytic mechanism are inferred based on modeling and their location.
 ==About this Structure==
-KA2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KA2 OCA].
+KA2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KA2 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Pyrococcus furiosus]]
 [[Category: Single protein]]
-[[Category: Arndt, J.W.]]
+[[Category: Arndt, J W.]]
-[[Category: Chan, M.K.]]
+[[Category: Chan, M K.]]
-[[Category: Chan, S.I.]]
+[[Category: Chan, S I.]]
 [[Category: Cheng, T.]]
 [[Category: Hao, B.]]
@@ Line 24: / Line 24: @@
 [[Category: metallopeptidase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:01:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:52 2008''