1g2r: Difference between revisions
New page: left|200px<br /><applet load="1g2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g2r, resolution 1.35Å" /> '''Structure of Cytosol... |
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[[Image:1g2r.gif|left|200px]]<br /><applet load="1g2r" size=" | [[Image:1g2r.gif|left|200px]]<br /><applet load="1g2r" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1g2r, resolution 1.35Å" /> | caption="1g2r, resolution 1.35Å" /> | ||
'''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue'''<br /> | '''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of the YlxR protein of unknown function from Streptococcus | The structure of the YlxR protein of unknown function from Streptococcus pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB operon in bacteria and belongs to a small protein family (COG2740) that shares a conserved sequence motif GRGA(Y/W). The family shows no significant amino-acid sequence similarity with other proteins. Three-wavelength diffraction MAD data were collected to 1.7 A from orthorhombic crystals using synchrotron radiation and the structure was determined using a semi-automated approach. The YlxR structure resembles a two-layer alpha/beta sandwich with the overall shape of a cylinder and shows no structural homology to proteins of known structure. Structural analysis revealed that the YlxR structure represents a new protein fold that belongs to the alpha-beta plait superfamily. The distribution of the electrostatic surface potential shows a large positively charged patch on one side of the protein, a feature often found in nucleic acid-binding proteins. Three sulfate ions bind to this positively charged surface. Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of binding sites and a large sharply bent cleft are observed in RNA-binding proteins that are unrelated in sequence and structure. It is proposed that YlxR is an RNA-binding protein. | ||
==About this Structure== | ==About this Structure== | ||
1G2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1G2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2R OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Gornicki, P.]] | [[Category: Gornicki, P.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: MCSG, Midwest | [[Category: MCSG, Midwest Center for Structural Genomics.]] | ||
[[Category: Maj, L.]] | [[Category: Maj, L.]] | ||
[[Category: Osipiuk, J.]] | [[Category: Osipiuk, J.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:24 2008'' | ||
