1vrq: Difference between revisions
New page: left|200px<br /><applet load="1vrq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vrq, resolution 2.20Å" /> '''Crystal Structure of... |
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[[Image:1vrq.gif|left|200px]]<br /><applet load="1vrq" size=" | [[Image:1vrq.gif|left|200px]]<br /><applet load="1vrq" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1vrq, resolution 2.20Å" /> | caption="1vrq, resolution 2.20Å" /> | ||
'''Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid'''<br /> | '''Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid'''<br /> | ||
==Overview== | ==Overview== | ||
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric | Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The alpha subunit is composed of two domains, contains NAD(+), and binds folinic acid. The beta subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10A apart. The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure. The delta subunit contains a single atom of zinc and has a Cys(3)His zinc finger structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine oxidase is discussed. | ||
==About this Structure== | ==About this Structure== | ||
1VRQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.] with ZN, SO4, FAD, NAD, FON, FMN and DMG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Sarcosine_oxidase Sarcosine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.1 1.5.3.1] Full crystallographic information is available from [http:// | 1VRQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=NAD:'>NAD</scene>, <scene name='pdbligand=FON:'>FON</scene>, <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=DMG:'>DMG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Sarcosine_oxidase Sarcosine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.1 1.5.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: heterotetrameric sarcosine oxidase]] | [[Category: heterotetrameric sarcosine oxidase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:55 2008'' | ||
Revision as of 16:37, 21 February 2008
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Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid
OverviewOverview
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The alpha subunit is composed of two domains, contains NAD(+), and binds folinic acid. The beta subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10A apart. The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure. The delta subunit contains a single atom of zinc and has a Cys(3)His zinc finger structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine oxidase is discussed.
About this StructureAbout this Structure
1VRQ is a Protein complex structure of sequences from Corynebacterium sp. with , , , , , and as ligands. Active as Sarcosine oxidase, with EC number 1.5.3.1 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96., Ida K, Moriguchi T, Suzuki H, Biochem Biophys Res Commun. 2005 Jul 29;333(2):359-66. PMID:15946648
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