1rfj: Difference between revisions
New page: left|200px<br /><applet load="1rfj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rfj, resolution 2.00Å" /> '''Crystal Structure of... |
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[[Image:1rfj.gif|left|200px]]<br /><applet load="1rfj" size=" | [[Image:1rfj.gif|left|200px]]<br /><applet load="1rfj" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rfj, resolution 2.00Å" /> | caption="1rfj, resolution 2.00Å" /> | ||
'''Crystal Structure of Potato Calmodulin PCM6'''<br /> | '''Crystal Structure of Potato Calmodulin PCM6'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of a potato calmodulin (PCM6) was solved by | The crystal structure of a potato calmodulin (PCM6) was solved by molecular replacement and refined to a crystallographic R factor of 22.8% (R(free) = 25.0%) using X-ray diffraction data in the resolution range 8.0-2.0 A. This is the first report of the three-dimensional structure of a plant Ca(2+)-calmodulin. PCM6 crystallizes in a crystal form that belongs to space group P2(1)2(1)2(1), which is different to that of most other calmodulin crystals. The main structural difference between PCM6 and the other calmodulins is in the central helix region and appears to be caused by crystal packing. The surface properties of PCM6 molecules were compared with those of animal calmodulins, which provided an explanation for the unique crystal-packing state of PCM6. | ||
==About this Structure== | ==About this Structure== | ||
1RFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1RFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Solanum tuberosum]] | [[Category: Solanum tuberosum]] | ||
[[Category: Chang, W | [[Category: Chang, W R.]] | ||
[[Category: Liang, D | [[Category: Liang, D C.]] | ||
[[Category: Yun, C | [[Category: Yun, C H.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: MPD]] | [[Category: MPD]] | ||
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[[Category: plant calmodulin]] | [[Category: plant calmodulin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:19 2008'' | ||
Revision as of 15:50, 21 February 2008
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Crystal Structure of Potato Calmodulin PCM6
OverviewOverview
The crystal structure of a potato calmodulin (PCM6) was solved by molecular replacement and refined to a crystallographic R factor of 22.8% (R(free) = 25.0%) using X-ray diffraction data in the resolution range 8.0-2.0 A. This is the first report of the three-dimensional structure of a plant Ca(2+)-calmodulin. PCM6 crystallizes in a crystal form that belongs to space group P2(1)2(1)2(1), which is different to that of most other calmodulin crystals. The main structural difference between PCM6 and the other calmodulins is in the central helix region and appears to be caused by crystal packing. The surface properties of PCM6 molecules were compared with those of animal calmodulins, which provided an explanation for the unique crystal-packing state of PCM6.
About this StructureAbout this Structure
1RFJ is a Single protein structure of sequence from Solanum tuberosum with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of potato calmodulin PCM6: the first report of the three-dimensional structure of a plant calmodulin., Yun CH, Bai J, Sun DY, Cui DF, Chang WR, Liang DC, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1214-9. Epub 2004, Jun 22. PMID:15213382
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