1n3o: Difference between revisions
New page: left|200px<br /><applet load="1n3o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3o, resolution 2.0Å" /> '''Pterocarcpus angolens... |
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[[Image:1n3o.gif|left|200px]]<br /><applet load="1n3o" size=" | [[Image:1n3o.gif|left|200px]]<br /><applet load="1n3o" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1n3o, resolution 2.0Å" /> | caption="1n3o, resolution 2.0Å" /> | ||
'''Pterocarcpus angolensis lectin in complex with alpha-methyl glucose'''<br /> | '''Pterocarcpus angolensis lectin in complex with alpha-methyl glucose'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus | The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site. | ||
==About this Structure== | ==About this Structure== | ||
1N3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis] with GYP, MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1N3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis] with <scene name='pdbligand=GYP:'>GYP</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3O OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Bouckaert, J.]] | [[Category: Bouckaert, J.]] | ||
[[Category: Buts, L.]] | [[Category: Buts, L.]] | ||
[[Category: Driessche, E | [[Category: Driessche, E Van.]] | ||
[[Category: Greve, H | [[Category: Greve, H De.]] | ||
[[Category: Imberty, A.]] | [[Category: Imberty, A.]] | ||
[[Category: Loris, R.]] | [[Category: Loris, R.]] | ||
[[Category: Read, J | [[Category: Read, J S.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:52 2008'' | ||
Revision as of 15:01, 21 February 2008
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Pterocarcpus angolensis lectin in complex with alpha-methyl glucose
OverviewOverview
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site.
About this StructureAbout this Structure
1N3O is a Single protein structure of sequence from Pterocarpus angolensis with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:12595543
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