2boy: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2BOY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhodococcus_opacus Rhodococcus opacus]] with FE, MG, BHO and LPP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | 2BOY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhodococcus_opacus Rhodococcus opacus]] with FE, MG, BHO and LPP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Catechol_1,2-dioxygenase Catechol 1,2-dioxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.1 1.13.11.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BOY OCA]]. | ||
==Reference== | ==Reference== | ||
Crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the Gram-positive Rhodococcus opacus 1CP grown on 2-chlorophenol., Ferraroni M, Kolomytseva MP, Solyanikova IP, Scozzafava A, Golovleva LA, Briganti F, J Mol Biol. 2006 Jul 21;360(4):788-99. Epub 2006 Jun 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16793061 16793061] | Crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the Gram-positive Rhodococcus opacus 1CP grown on 2-chlorophenol., Ferraroni M, Kolomytseva MP, Solyanikova IP, Scozzafava A, Golovleva LA, Briganti F, J Mol Biol. 2006 Jul 21;360(4):788-99. Epub 2006 Jun 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16793061 16793061] | ||
[[Category: Catechol 1,2-dioxygenase]] | |||
[[Category: Rhodococcus opacus]] | [[Category: Rhodococcus opacus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:03:42 2007'' | ||
Revision as of 10:59, 30 October 2007
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CRYSTAL STRUCTURE OF 3-CHLOROCATECHOL 1,2-DIOXYGENASE FROM RHODOCOCCUS OPACUS 1CP
OverviewOverview
The crystal structure of the 3-chlorocatechol 1,2-dioxygenase from the, Gram-positive bacterium Rhodococcus opacus (erythropolis) 1CP, a Fe(III), ion-containing enzyme specialized in the aerobic biodegradation of, 3-chloro- and methyl-substituted catechols, has been solved by molecular, replacement techniques using the coordinates of 4-chlorocatechol, 1,2-dioxygenase from the same organism (PDB code 1S9A) as a starting model, and refined at 1.9 A resolution (R(free) 21.9%; R-factor 17.4%). The, analysis of the structure and of the kinetic parameters for a series of, different substrates, and the comparison with the corresponding data for, the 4-chlorocatechol 1,2-dioxygenase isolated from the same bacterial, strain, provides evidence of which active site residues are responsible, for the ... [(full description)]
About this StructureAbout this Structure
2BOY is a [Single protein] structure of sequence from [Rhodococcus opacus] with FE, MG, BHO and LPP as [ligands]. Active as [Catechol 1,2-dioxygenase], with EC number [1.13.11.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the Gram-positive Rhodococcus opacus 1CP grown on 2-chlorophenol., Ferraroni M, Kolomytseva MP, Solyanikova IP, Scozzafava A, Golovleva LA, Briganti F, J Mol Biol. 2006 Jul 21;360(4):788-99. Epub 2006 Jun 5. PMID:16793061
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