2igc: Difference between revisions
New page: left|200px<br /><applet load="2igc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2igc, resolution 1.40Å" /> '''Structure of Spin la... |
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[[Image:2igc.jpg|left|200px]]<br /><applet load="2igc" size=" | [[Image:2igc.jpg|left|200px]]<br /><applet load="2igc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2igc, resolution 1.40Å" /> | caption="2igc, resolution 1.40Å" /> | ||
'''Structure of Spin labeled T4 Lysozyme Mutant T115R1A'''<br /> | '''Structure of Spin labeled T4 Lysozyme Mutant T115R1A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2IGC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 2IGC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: t4 lysozyme]] | [[Category: t4 lysozyme]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:54:39 2008'' | ||
Revision as of 15:54, 23 January 2008
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Structure of Spin labeled T4 Lysozyme Mutant T115R1A
OverviewOverview
A nitroxide side chain (R1) has been substituted at single sites along a, helix-turn-helix motif in T4 lysozyme (residues 114-135). Together with, previously published data, the new sites reported complete a continuous, scan through the motif. Mutants with R1 at sites 115 and 118 were selected, for crystallographic analysis to identify the structural origins of the, corresponding two-component EPR spectra. At 115, R1 is shown to occupy two, rotamers in the room temperature crystal structure, one of which has not, been previously reported. The two components in the EPR spectrum, apparently arise from differential interactions of the two rotamers with, the surrounding structure, the most important of which is a hydrophobic, interaction of the nitroxide ring. Interestingly, the crystal structure at, 100 K reveals a single rotamer, emphasizing the possibility of rotamer, selection in low-temperature crystal structures. Residue 118 is at a, solvent-inaccessible site in the protein core, and the structure of 118R1, the first reported for the R1 side chain at a buried site, reveals how the, side chain is accommodated in an overpacked core.
About this StructureAbout this Structure
2IGC is a Single protein structure of sequence from Bacteriophage t4. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
ReferenceReference
Structural determinants of nitroxide motion in spin-labeled proteins: Tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme., Guo Z, Cascio D, Hideg K, Kalai T, Hubbell WL, Protein Sci. 2007 Jun;16(6):1069-86. Epub 2007 May 1. PMID:17473014
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